Figure 4

Responses of atsrp2 and atsrp3 mutants to MMS. B5 and half-strength MS (1/2 MS) media were used for seedling growth. (A) Vigour of atsrp2 and atsrp3 mutants compared to wild-type following treatment with MMS. Seedlings (5-d) of equal size were grown in 1/2 MS liquid media containing the indicated concentrations of MMS for 2 weeks. Size bars = 10 mm. (B-E) Seedlings (wild-type and mutants) were grown in the presence of the indicated concentrations of MMS on single plates to provide identical growth conditions. (B) Wild-type and mutant plants showing differential root lengths. Seedlings (5-d) were grown on B5 solid media. Arrows indicate root tips of mutants and wild-type plants (centre plant in each case) for comparison of root lengths. Photographs were taken on day 14. (C) Root lengths. Values are means +/- SE (n = 10). Seedlings (5-d) were grownon 1/2 MS solid media. (D, E) Seedlings (2-week) were transplanted to B5 solid media containing 55 ppm MMS. (D) Leaf number per plant. Counts were made at 4 weeks after transplantation. Values are means +/- SE (n = 4). (E) Wild-type and mutant plants showing different plant sizes. Photographs were taken 4 weeks after transplantation. Size bars = 10 mm.