Figure 3

Schematic representations of atsrp2 and atsrp3 T-DNA insertion mutants and identification of homozygous lines. (A, B) Genomic sequences of AtSRP3 and AtSRP2 each contain two exons. Primers for screening of homozygous lines are indicated by letters a-f and LB (left border). Gray boxes indicate the RCL motif. (C, D) Genomic-PCR analysis for screening of homozygous lines using the primers shown in A and B. wt, wild-type. (E) Confirmation of knockout of AtSRP2 and AtSRP3 expression in each mutant via semi-quantitative RT-PCR. RNA was isolated from developing siliques. Values in brackets indicate number of cycles. Actin2 served as a control. (F) Phenotype of atsrp2 and atsrp3 mutants. Mutants and wild-type plants were grown in a growth chamber at 24°C. Photographs were taken at 4 weeks after sowing.