Figure 2

qRT-PCR analyses of miR172 target genes in wild-type plants. A primer pair spanning the miR172 target site was used to quantify expression of the uncleaved target mRNAs. For each gene, relative fold expression difference is shown by using the expression level detected in flag leaf as the reference. Error bars represent standard deviation of the expression ratio. 2L-S and 2L-R: shoot and root of two-leaf stage seedlings. 4L: the 4^th leaf. 10L: the 10^th leaf. 10L-SA: shoot apex of 10-leaf stage seedlings. 10L-R: 10-leaf stage root. FL: flag leaf. ≤ 0.5P, 0.5-1P, 1-2P and 2-4P: developing panicles with a length of ≤ 0.5 cm, 0.5-1 cm, 1-2 cm and 2-4 cm, respectively. BP: booting panicle. Em: embryo. En: endosperm.