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Table 1 The mutation report of Mutation Surveyor® after sequence trace analysis of mutant/non-mutant mixed samples.

From: Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®

Mutant allele in pooled DNA

Sample File

Reference File

Direction

Mutation *

Score

1/2

B11F_D01.ab1

Q7D3_F_G08.ab1

Forward

(352)G>GA$20

20

1/2

B11R_C01.ab1

Q7D3_R_G09.ab1

Reverse

(392)G>GA$43

43

1/4

B21F_D02.ab1

Q7D3_F_G08.ab1

Forward

(352)G>GA$9

9

1/4

B21R_C02.ab1

Q7D3_R_G09.ab1

Reverse

(392)G>GA$16

16

1/6

B31F_D03.ab1

Q7D3_F_G08.ab1

Forward

(352)G>AG$7

7

1/6

B31R_C03.ab1

Q7D3_R_G09.ab1

Reverse

(392)G>AG$7

7

1/8

B41F_D04.ab1

Q7D3_F_G08.ab1

Forward

(352)G>AG$7

7

1/8

B41R_C04.ab1

Q7D3_R_G09.ab1

Reverse

(392)G>AG$7

7

1/10

B51F_D05.ab1

Q7D3_F_G08.ab1

Forward

(352)G>AG$7

7

1/10

B51R_C05.ab1

Q7D3_R_G09.ab1

Reverse

(392)G>AG$7

7

1/12

B61F_D06.ab1

Q7D3_F_G08.ab1

Forward

n.a.

n.a.

1/12

B61R_C06.ab1

Q7D3_R_G09.ab1

Reverse

n.a.

n.a.

  1. *Mutation report indicates the position (in brackets), the base change (G>GA) and the score (the number after the $ sign).