Figure 2From: Rapid and dynamic subcellular reorganization following mechanical stimulation of Arabidopsisepidermal cells mimics responses to fungal and oomycete attackFormation of an actin patch beneath the contact site. Actin microfilaments visualized in the cortical cytoplasm underlying the outer epidermal cell wall in a cotyledon of A. thaliana expressing hTalin-GFP. The surface of the epidermal cell was touched with a glass microneedle at time 0:00 at the site indicated by the asterisk in A. Images A-J are projections of six optical sections taken at the times indicated in minutes and seconds. Actin microfilaments began to concentrate beneath the needle contact site 1 minute 58 seconds after touching the epidermal cell surface. The patch of actin continued to enlarge over the ensuing hour. The arrows in A and J indicate a thick actin cable that remains in the same position throughout the 51-minute sequence. A movie composed of images from 94 time points taken during this time is shown in Additional File 1. Bar = 10 μm.Back to article page