Primer Id | C. arabica (n = 8) | C. canephora (n = 8) | Coffea spp. (n = 12) | Psilanthus spp. (n = 2) | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 | NA | PA$ | Allele range | Ho | He | PIC | NA | PA$ | Allele range | Ho | He | PIC | NA | PA$ | Allele range | NA | PA$ | Allele range |
CaM02 | 2 | 0 | 252–262 | Duplicated loci | 2 | 0 | 256–268 | 0.71 | 0.69 | 0.67 | 8 | 4a,c,k,l | 252–278 | 2 | 0 | 262–272 | ||
CaM03 | 6 | 16 | 164–184 | 0.38 | 0.74* | 0.70 | 6 | 0 | 171–194 | 0.63 | 0.88 | 0.83 | 12 | 5c,e,f,j,l | 165–201 | 1 | 1m | 187 |
CaM06 | 3 | 0 | 285–327 | Duplicated loci | 2 | 0 | 275–277 | 1.00 | 0.53* | 0.56 | 4 | 1j | 275–289 | 2 | 0 | 275–281 | ||
CaM08 | 1 | 0 | 210 | Monomorphic | 3 | 116 | 201–205 | 0.50 | 0.43 | 0.40 | 4 | 2b,l | 142–201 | 1 | 1n | 254 | ||
CaM09 | 1 | 0 | 135 | Monomorphic | 3 | 116 | 135–139 | 0.50 | 0.43 | 0.40 | 7 | 5a,b,g,l | 124–211 | NA | -- | -- | ||
CaM11 | 1 | 0 | 286 | Monomorphic | 1 | 0 | 286 | Monomorphic | 4 | 2c,h | 278–295 | NA | -- | -- | ||||
CaM12 | 1 | 0 | 137 | Monomorphic | 4 | 110 | 122–137 | 1.00 | 0.65** | 0.61 | 4 | 1g | 124–137 | 2 | 0 | 131–137 | ||
CaM13 | 2 | 15 | 281–286 | 0.00 | 0.23 | 0.23 | 1 | 0 | 286 | Monomorphic | 7 | 3j,k | 278–336 | 2 | 1n | 255–283 | ||
CaM15 | 2 | 0 | 167–170 | Duplicated loci | 1 | 0 | 167 | Monomorphic | 2 | 1l | 164–167 | 2 | 2m,n | 153–156 | ||||
CaM16 | 3 | 0 | 187–198 | 0.63 | 0.51 | 0.49 | 4 | 111 | 181–198 | 0.75 | 0.74 | 0.72 | 9 | 2c,l | 177–198 | 2 | 0 | 191–193 |
CaM17 | 2 | 0 | 175–181 | 0.88 | 0.53 | 0.55 | 2 | 0 | 175–181 | 0.63 | 0.46 | 0.48 | 3 | 1l | 162–181 | 2 | 0 | 175–181 |
CaM18 | 5 | 0 | 180–189 | Duplicated loci | 5 | 0 | 178–186 | 0.38 | 0.79** | 0.75 | 12 | 5d,e,j,k | 174–189 | 1 | 0 | 175 | ||
CaM20 | 2 | 0 | 184–192 | 0.13 | 0.46 | 0.48 | 3 | 0 | 192–200 | 0.13 | 0.42* | 0.40 | 3 | 1d | 192–198 | NA | -- | -- |
CaM21 | 2 | 0 | 158–164 | Duplicated loci | 4 | 110 | 158–162 | 0.25 | 0.64** | 0.62 | 9 | 3a,j,l | 154–178 | 3 | 3m,n | 161–172 | ||
CaM22 | 1 | 0 | 103 | Monomorphic | 6 | 29,16 | 99–110 | 0.43 | 0.86** | 0.80 | 8 | 2c,l | 82–122 | 1 | 0 | 88 | ||
CaM23 | 1 | 0 | 154 | Monomorphic | 1 | 0 | 154 | Monomorphic | 3 | 1l | 140 154 | 2 | 2m,n | 152–158 | ||||
CaM24 | 2 | 0 | 191–197 | 0.00 | 0.50** | 0.52 | 4 | 0 | 191–198 | 0.43 | 0.71 | 0.67 | 8 | 4a,b,g,l | 178–204 | 2 | 2m,n | 177–189 |
CaM25 | 4 | 0 | 182–185 | 0.13 | 0.53** | 0.5 | 3 | 0 | 182–184 | 0.63 | 0.51 | 0.48 | 5 | 0 | 182–186 | 2 | 0 | 182–186 |
CaM26 | 3 | 0 | 252–259 | 0.00 | 0.43** | 0.42 | 5 | 0 | 247–255 | 0.25 | 0.80** | 0.76 | 11 | 3g,h,k | 241–262 | 1 | 0 | 254 |
CaM27 | 3 | 0 | 150–169 | 0.13 | 0.34 | 0.33 | 3 | 0 | 161–169 | 0.88 | 0.68 | 0.64 | 8 | 2a,c | 150–169 | 2 | 0 | 161–168 |
CaM30 | 2 | 0 | 216–229 | 0.13 | 0.13 | 0.12 | 2 | 0 | 216–218 | 0.63 | 0.46 | 0.48 | 7 | 2f,j | 212–229 | 4 | 3m,n | 210–225 |
CaM31 | 3 | 0 | 258–261 | Duplicated loci | 4 | 0 | 258–262 | 0.38 | 0.59 | 0.57 | 6 | 2h,k | 258–267 | 1 | 1m | 265 | ||
CaM32 | 4 | 0 | 127–145 | 0.88 | 0.69 | 0.68 | 5 | 114 | 145–158 | 0.75 | 0.72 | 0.68 | 10 | 2a,e | 127–164 | 3 | 1m | 133–145 |
CaM33 | 3 | 21,6 | 230–233 | 0.13 | 0.34 | 0.32 | 7 | 212,13 | 226–241 | 0.71 | 0.88 | 0.83 | 11 | 5b,d,f,i,k | 213–143 | 1 | 1m | 217 |
CaM34 | 2 | 0 | 194–199 | Duplicated loci | 2 | 0 | 198–200 | 0.00 | 0.23 | 0.23 | 5 | 2e,l | 194–209 | 2 | 2m,n | 166–171 | ||
CaM35 | 3 | 0 | 192–211 | Duplicated loci | 4 | 0 | 198–211 | 0.63 | 0.69 | 0.66 | 7 | 1g | 186–211 | 1 | 0 | 204 | ||
CaM36 | 5 | 33,7,8 | 228–253 | 0.00 | 0.85** | 0.78 | 7 | 6except 10,15 | 230–268 | 0.17 | 0.92** | 0.86 | 10 | 8 a,c,e,f,h,i,h,l | 181–262 | 1 | 1n | 190 |
CaM38 | 6 | 14 | 214–226 | 0.38 | 0.86** | 0.81 | 5 | 29,10,12,15 | 227–235 | 0.17 | 0.80** | 0.74 | 6 | 2b,d | 220–241 | 2 | 0 | 223–227 |
CaM39 | 2 | 0 | 174–186 | Duplicated loci | 3 | 0 | 180–194 | 1.00 | 0.59* | 0.60 | 9 | 2b,h | 174–205 | 3 | 3m,n | 208–229 | ||
CaM40 | 5 | 15 | 230–240 | 0.40 | 0.82 | 0.75 | 7 | 116 | 226–242 | 0.50 | 0.91* | 0.86 | 8 | 2d,e | 232–246 | 3 | 0 | 233–239 |
CaM41 | 6 | 45,6,8 | 232–243 | 0.25 | 0.68** | 0.65 | 5 | 19 | 234–242 | 0.25 | 0.81** | 0.77 | 4 | 0 | 235–242 | 2 | 1n | 237–244 |
CaM42 | 2 | 0 | 192–196 | 0.75 | 0.50 | 0.52 | 2 | 0 | 190–192 | 0.00 | 0.53* | 0.56 | 7 | 2e,l | 173–199 | 2 | 2m,n | 191–195 |
CaM43 | 3 | 0 | 196–211 | Duplicated loci | 4 | 211,15 | 198–203 | 0.63 | 0.64 | 0.64 | 10 | 4b,c,e,f | 188–224 | 2 | 1n | 192–196 | ||
CaM44 | 2 | 16 | 215–217 | 0.00 | 0.23 | 0.23 | 2 | 0 | 224–226 | 0.00 | 0.23 | 0.23 | 10 | 3c,h,l | 194–227 | 3 | 1n | 221–227 |
CaM45 | 3 | 0 | 151–182 | 0.50 | 0.43 | 0.42 | 5 | 210,13 | 151–235 | 0.75 | 0.6 | 0.57 | 8 | 3d,i,k | 147–214 | 3 | 1m | 145–193 |
CaM46 | 4 | 23,6 | 208–228 | 0.38 | 0.69 | 0.65 | 6 | 114 | 208–223 | 0.38 | 0.82** | 0.78 | 7 | 1e | 208–234 | 2 | 1n | 208–212 |
CaM49 | 3 | 0 | 191–194 | 0.38 | 0.68** | 0.66 | 4 | 0 | 190–194 | 0.71 | 0.76 | 0.72 | 8 | 3g,j,k | 186–194 | NA | -- | -- |
CaM52 | 2 | 0 | 157–159 | 0.00 | 0.23 | 0.23 | 3 | 0 | 148–158 | 0.13 | 0.34 | 0.33 | 5 | 0 | 148–158 | 1 | 0 | 155 |
CaM53 | 1 | 0 | 172 | Monomorphic | 3 | 22 | 167–190 | 0.13 | 0.24 | 0.23 | 5 | 3i,j,l | 125–197 | 2 | 2m,n | 170–184 | ||
CaM54 |  |  |  | No amplification | 2 | 0 | 176–184 | 0.57 | 0.44 | 0.45 | 1 | 0 | 184 | 1 | 1n | 164 | ||
CaM55 | 2 | 0 | 144–151 | Duplicated loci | 6 | 215,16 | 159–178 | 0.75 | 0.84 | 0.79 | 9 | 0 | 144–178 | 1 | 0 | 160 | ||
CaM57 | 3 | 0 | 146–188 | Duplicated loci | 5 | 0 | 102–176 | 0.63 | 0.77 | 0.73 | 9 | 2a,l | 102–174 | 3 | 1m | 102–156 | ||
CaM58 | 2 | 0 | 189–191 | Duplicated loci | 2 | 0 | 183–191 | 0.75 | 0.5 | 0.52 | 8 | 2b,l | 181–224 | 2 | 0 | 192–193 | ||
CaM59 | 2 | 0 | 224–226 | 0.13 | 0.13 | 0.12 | 3 | 0 | 222 –225 | 0.88 | 0.69 | 0.67 | 4 | 0 | 222–228 | 3 | 0 | 222–228 |
Range | 0–6 | 0–4 | -- | 0–0.88 | 0.13–0.86 | 0.12–0.81 | 1–7 | 0–6 | -- | 0–1.00 | 0.23–0.88 | 0.23–0.83 | 1–13 | 0–8 | -- | 0–4 | 0–3 | -- |
Mean | 2.7 | 0.37 | -- | 0.29 | 0.5 | 0.49 | 3.78 | 0.67 | -- | 0.52 | 0.63 | 0.62 | 7.07 | 2.42 | -- | 1.98 | 0.87 | -- |
SD (±) | 1.4 | 0.87 | -- | 0.28 | 0.22 | 0.21 | 1.73 | 1.13 | -- | 0.29 | 0.19 | 0.18 | 2.87 | 1.72 | -- | 0.79 | 0.95 | -- |
SE (±) | 0.3 | 0.13 | -- | 0.06 | 0.05 | 0.04 | 0.26 | 0.17 | -- | 0.04 | 0.03 | 0.03 | 0.43 | 0.26 | -- | 0.12 | 0.14 | -- |