Characterisation of the tryptophan synthase alpha subunit in maize
© Kriechbaumer et al; licensee BioMed Central Ltd. 2008
Received: 06 February 2008
Accepted: 22 April 2008
Published: 22 April 2008
In bacteria, such as Salmonella typhimurium, tryptophan is synthesized from indole-3-glycerole phosphate (IGP) by a tryptophan synthase αββα heterotetramer. Plants have evolved multiple α (TSA) and β (TSB) homologs, which have probably diverged in biological function and their ability of subunit interaction. There is some evidence for a tryptophan synthase (TS) complex in Arabidopsis. On the other hand maize (Zea mays) expresses the TSA-homologs BX1 and IGL that efficiently cleave IGP, independent of interaction with TSB.
In order to clarify, how tryptophan is synthesized in maize, two TSA homologs, hitherto uncharacterized ZmTSA and ZmTSAlike, were functionally analyzed. ZmTSA is localized in plastids, the major site of tryptophan biosynthesis in plants. It catalyzes the tryptophan synthase α-reaction (cleavage of IGP), and forms a tryptophan synthase complex with ZmTSB1 in vitro. The catalytic efficiency of the α-reaction is strongly enhanced upon complex formation. A 160 kD tryptophan synthase complex was partially purified from maize leaves and ZmTSA was identified as native α-subunit of this complex by mass spectrometry. ZmTSAlike, for which no in vitro activity was detected, is localized in the cytosol. ZmTSAlike, BX1, and IGL were not detectable in the native tryptophan synthase complex in leaves.
It was demonstrated in vivo and in vitro that maize forms a tryptophan synthase complex and ZmTSA functions as α-subunit in this complex.
Tryptophan is an essential amino acid for human nutrition. In kernels of cereals, e.g. maize (Zea mays), the tryptophan content is low, limiting the nutritional value. Significant effort is made to breed maize lines with enhanced tryptophan content [1, 2]. In addition to its function as protein component, plants utilize tryptophan as precursor of a large variety of secondary metabolites like terpenoid indole alkaloids, indole glucosinolates, and indolic phytoalexins (reviewed in: [3, 4]). Of special importance is the tryptophan-derived plant hormone indole-3-acetic acid (IAA), which is involved in numerous processes, including embryo development, apical dominance, and tropisms [4, 5]. These essential functions of tryptophan emphasize the need to understand its synthesis in plants in more detail.
The picture is more complex in higher plants. The homologs of the bacterial TSA and TSB genes are generally duplicated, e.g. the Arabidopsis thaliana genome contains two putative TSA and four putative TSB genes. Currently the role of these different isoforms is not fully understood. Functional relevance of AtTSA1 (At3g54640) and AtTSB1 (At5g54810) was demonstrated by the facultative tryptophan auxotroph mutants trp3 and trp2, respectively [11, 12]. AtTSA1 and AtTSB1 are both localized in the plastid [11, 13, 14]. Based on immunoaffinity chromatography it was strongly suggested that the two proteins form an active αβ complex . However, it is not known whether formation of such complexes is a general phenomenon in plants.
Among the cereals most information on TSA and TSB homologs is available for maize: Two highly similar TSB genes (ZmTSB1 and ZmTSB2) have been identified, sharing 96% identity on the mature protein level. ZmTSB1 and ZmTSB2 are functionally redundant active TSB enzymes. While single mutations in either gene do not affect tryptophan synthesis, the double mutant orange pericarp (orp1 = Zmtsb1, orp2 = Zmtsb2) is tryptophan auxotroph [15, 16]. Four TSA homologs are present: BX1, IGL, ZmTSAlike , and ZmTSA (this work). BX1 is essential for providing indole as precursor of the natural pesticide 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA) . The 3-dimensional BX1 structure has been determined and shown to be very similar to the active conformation of bacterial TSAs . Igl transcription is triggered by insect feeding and IGL provides indole as a volatile signal for parasitic wasps [17, 20]. BX1 and IGL efficiently cleave IGP to form indole, while the activity of bacterial TSA subunits is dependent on the interaction with a β-subunit [17, 18]. These enzymatic properties, their specific transcriptional regulation, and the lack of growth defects of bx1 and igl mutant plants suggested that additional TSA-homologs are involved in tryptophan biosynthesis.
In this study we aimed to identify the TSA homolog from maize that is involved in tryptophan biosynthesis. ZmTSA is catalytically active and interaction of ZmTSA with a β-subunit strongly enhances the catalytic efficiency of the α-reaction. A protein purification strategy was applied to obtain direct evidence that angiosperms, similarly to bacteria, form a tryptophan synthase complex.
Plant material and growth conditions
The following maize (Zea mays) lines were analyzed: B73 (wildtype), bx1 mutant , and the tsb mutants (orp1 +/orp1 orp2) and (orp1 orp2/+ orp2)  that were kindly provided by the maize genetic stock center. Seedlings were germinated in a beaker rolled in wet filter paper (603/N, 75 g m-2, Sartorius) at 28°C in the dark and after three days the seedlings were transferred to soil and incubated in a Heraeus HPS 2000 growth chamber (light: 100 μmol m-2 s-1; 16 h/d).
Identification of ZmTSA and heterologous expression of standard proteins
For isolation of cDNAs a library prepared from 12 day old seedlings, line bx1  was used . A ZmTSAlike cDNA clone was isolated based on the genomic sequence  and confirmed to be full-length by RACE. The ZmTSA cDNA was cloned [GenBank:EU334442] based on the EST AY107255 (gene bank) and the EST-TUG Zmtuc03-08-11.4557 (maize genomic database) and confirmed to be full-length based on the genomic sequence. ZmTSA and ZmTSAlike were analyzed for plastid targeting sequences using the programs "TargetP" and "iPSORT" [22, 23].
BX1 and IGL expression and purification have been described previously [17, 18]. For heterologous expression of ZmTSA and ZmTSAlike, an NdeI/BglII-fragment, for ZmTSB1 expression an NdeI/BamHI-fragment of the coding sequence excluding plastid-targeting sequences was amplified by PCR. The following primers were used: ZmTSA: GCATATGCCGCGCAGCATCTCCG, TCTTACGCTCTTTGCTAACGAAAATGG; ZmTSAlike: CGCATATGGCCAACGGCGGCG, GGGAGTGAGATCTGCTCACGGC; ZmTSB1: CATATGGCGGCCTCCCCCGCTGCCG, CTCGGATCCAGCCCTCCTCTCCGGTG. The coding sequences were cloned into pET28a His-tag vector, heterologously expressed, and purified under native conditions by His-tag affinity purification via Ni-NTA agarose according to the manufacturers' suggestions (Qiagen, Hilden, Germany).
For the detection of TSA/TSB complex formation in vitro, size exclusion chromatography (HiLoad™16/60 Superdex™200 prep grade, Amersham Biosciences, Little Chalfont, UK) was performed using 100 mM Tris-HCl, pH 8.0, 100 mM KCl at 0.5 ml min-1 and 20°C. The column was calibrated using the protein standards cytochrome c (12.4 kD), carbonic anhydrase (29 kD), bovine albumin (66 kD), alcohol dehydrogenase (150 kD), and β-amylase (200 kD).
For detection of ZmTSA and ZmTSAlike expression total RNA was isolated from the wildtype line B73 and quantitative real time PCR was carried out using the LightCycler/Syb®-Green dye system (Roche, Mannheim, Germany) with the following primer pairs: ZmTSA: CACTGCTGGAGACCCTGACT, GGTTCATGGCAATGCGGCCT; ZmTSAlike: CCACAAAGGCAGCGCTCGGAGGTG, GCCTCGCTCCTCAGCAACGTCGTCT; GAPDH C: GCTAGCTGCACCACAAACTGCCT, TAGCCCCACTCGTTGTCGTACCA.
Tissues analyzed are the following: leaf from 12 d old plants (12 d leaf), 12 d leaf methyl jasmonate treated, 12 d leaf elicitor treated , 4 d shoot dark grown, 6 d shoot dark grown, 6 d shoot light grown, 3 week root, 10 week crown root, 8 week stem, 10 week leaf, husk, silk, cob, tassel, kernel 1 week after pollination (wap), kernel 3 wap.
Tryptophan synthase activity assays
Plant protein fractions (200 μg) or purified recombinant enzyme (2 μg) were incubated 3 h for plant protein, 5 min for recombinant proteins, respectively, at 30°C, in 80 mM potassium phosphate buffer, pH 8.2 containing the following substrates: α-reaction: 100 μM IGP ; β-reaction: 50 μM indole, 60 mM L-serine, 50 μM pyridoxal phosphate; αβ-reaction: 100 μM IGP, 60 mM L-serine, 50 μM pyridoxal phosphate, concentration ranges were analyzed for determination of kinetic parameters.
The products indole and tryptophan were quantified by HPLC (RP-column: LiChroCART 125–4, RP-18, 5 μm; Merck, West Point, PA) using diode array (PDA-100, Dionex, Idstein, Germany) and fluorescence detection (RF-10AXL, Shimadzu, Duisburg, Germany; excitation: 285 nm, emission: 360 nm). The mobile phase was delivered with a flow rate of 1 ml min-1 with an initial mixture of 15% (v/v) MeOH in 0.3% (v/v) HCOOH followed by a 15 min linear gradient to 100% MeOH.
Plant protein purification
Leaf tissue (50 g) was homogenized in liquid nitrogen and extracted in 5 ml 50 mM Tris-HCl, pH 8.0, containing 10 mM EDTA, 5 mM DTE, 1 mM PMSF, and 10% Polyclar AT (Serva, Heidelberg, Germany) and centrifuged 20 min at 10.000 g (4°C). The supernatant was subjected to an anion exchange column (MonoQ HR 5/5, Amersham) equilibrated with 100 mM Tris-HCl, pH 8.0, 10 mM EDTA, 5 mM DTE, 100 mM NaCl at 4°C. The column was then washed with 10 Vol of the same buffer and eluted with 100 mM Tris-HCl, pH 8.0 containing 10 mM EDTA, 5 mM DTE, and 1 M NaCl in a 20 Vol linear gradient. Fractions around 450 mM NaCl showed TS activity and were subjected (0.5 ml min-1, 20°C) to gel permeation chromatography (HiLoad™16/60 Superdex™200 prep grade, Amersham, equilibrated with 100 mM Tris-HCl, pH 8.0 including 100 mM KCl). The column was eluted with the same buffer (180 ml, 0.5 ml min-1). 1 ml fractions were collected and tested for α- and β-activity. The 54 to 56 ml fractions were precipitated by addition of 10% TCA, redissolved in 10 μl 10 mM Tris-HCl, pH 6.8, 20 mM DTT, 2% (w/v) SDS, 0.01% (w/v) bromphenol blue, 10% (w/v) glycerol, and subjected to SDS-PAGE.
After fixation for > 2 h with a 40% (v/v) MeOH/10% (v/v) HOAc solution and washing in water for 2 × 10 min the gels were stained over night with Coomassie dye (0.08% (w/v) Coomassie Brilliant Blue G250, 1.6% (w/v) ortho-phosphoric acid, 8% (w/v) (NH4)2SO4, 20% (v/v) MeOH) and destained in 1% (v/v) HOAc . The protein bands between 25 and 60 kD were cut out and the gel piece was further destained in a thermo mixer with water (2 × 30 min 37°C), 200 mM NH4HCO3, pH 7.8 (2 × 30 min 37°C), and 50% acetonitrile (ACN) (2 × 5 min 37°C). The gel slice was shrunk in 100% ACN, the liquid supernatant was removed and the gel dried in a SpeedVac for 5 min.
Identification of tryptophan synthase protein components: sample preparation
Tryptic digestion: 100 μl trypsin solution (200 ng μl-1, Promega) were directly pipetted on the gel piece, incubated for 10 min on ice to allow the trypsin to move into the gel and then covered with 500 μl 25 mM NH4HCO3 followed by 16 h incubation at 37°C. Digestion was stopped by adding 50 μl of 10% trifluoroacetic acid and the supernatant was transferred to a new tube. Peptides were extracted by consecutive basic and acid extraction. Basic extraction: 50 μl 40 mM NH4HCO3 were added to the gel, shaken for 30 min at 37°C, and the supernatant transferred to a new tube. The same incubation followed after addition of 50 μl ACN. Both steps were repeated and the supernatants pooled. Acid extraction: The gel piece was extracted twice in 50 μl 5% (v/v) HCOOH for 30 min. The gel piece was shrunk twice in 50 μl ACN. All the collected supernatants were pooled, dried in a SpeedVac and dissolved in 100 μl 20 mM ammonium acetate buffer, pH 7.4, 10% (v/v) ACN, 5 mM DTT.
A further step of digestion was performed in solution (10 μl trypsin solution, 200 ng μl-1, 8 h, 37°C) to apply a maximum amount of hydrolyzed peptides without miscleavage. Latter is important for a reproducible identification of qualifying peptides.
Liquid chromatography – mass spectrometry (LC-MS)
An Agilent micro HPLC system (series 1100, Waldbronn, Germany) consisting of a quaternary capillary pump (G1376A), a degasser unit (G1379A), an auto sampler (G1377A), and a column in a thermostat set to 40°C (G1316A) was used in combination with a single time-of-flight mass spectrometer (LC/MSD TOF, Agilent Technologies, Santa Clara, USA). The chromatographic separation was performed with a Zorbax SB C18 column (150 × 0.5 mm i.d.; 5 μm, Agilent Technologies, Santa Clara, USA) by 8 μl sample injections. Prior to injection, the trypsinized protein samples were mixed with 200 μl 20 mM NH4Ac, 10% ACN (v/v), 5 mM DTT, pH 7.4, sonified for 15 min, filtrated via HV filter, and stored in an autosampler vial. The HPLC separation flow rate was 50 μl min-1. At the beginning of each chromatographic run, the composition of the mobile phase was kept at 95% 20 mM NH4Ac/5% ACN (v/v), following a gradient to 20% 20 mM NH4Ac/80% ACN (v/v) within 5 min and this final value was held for 15 min.
MS measurements were performed in positive ionization mode with the mass spectrometer equipped by an ESI source. The applied MS parameter were as follows: 350°C drying gas temperature, 420 Lh-1 drying gas flow rate, 20 psig nebulizer gas pressure, 4000 V capillary voltage, 60 V skimmer voltage and 215 V fragmentor voltage. The mass-range was set to 150 – 3200 m/z and data acquisition was 0.88 cycles/sec. The drying gas nitrogen was supplied by a nitrogen generator (nitrogen purity ≥ 99.5%, Domnick Hunter, Willich, Germany). The ChemStation software (Rev. B.01.01, Agilent, Waldbronn, Germany) was used for system control and the Analyst QS software (LC-MS TOF Software, Ver. A.01.00 (B663), June, 2004) for the data acquisition.
Expression of ZmTSA- and ZmTSAlike-GFP-fusion proteins
To construct vectors for expression of GFP-fusion protein , the stop codon of the ZmTSA coding sequence was replaced by a BglII restriction site, using the following primers: 5'-CGACTACACCAAATGAAAGAATGGAG-3' (forward), 5'-CTCGAGAGATCTGGCAATGCGGCCTTCAGG-3' (reverse). The full size ZmTSA cDNA fragment, in which the stop codon was eliminated, was then cut from the vector with EcoRI/BglII and cloned into the EcoRI/BamHI sites of the pEZS-NL vector (D. Ehrhardt, Carnegie Institution). The ZmTSA-eGFP chimera was cut with EcoRI, blunted, cut with XbaI, and cloned into the SmaI/XbaI sites of the PvuII-deletion of pPCVE35E plant transformation vector. The same strategy was used for the construction of the ZmTSAlike-eGFP chimeric cDNA, with the primer pair: CAAGCTGGCATACATGGAC/GGTACCAGATCTGGCATAGCAGCCTTCATA.
Transfection of maize protoplasts and confocal microscopy
Maize LG22 seedlings were grown on an 8 h dark, 20°C/16 h light, 26°C regime for 6 to 8 days and were then transferred to the dark for 3 days. Protoplasts were isolated from the second true leaves essentially as described previously [27, 28]. Digestion was performed in 1% (w/v) cellulase R10, 0,5% (w/v) Macerozyme R10 (both from Yakult Honsha), 0.6 M mannitol, 10 mM MES, pH 5.7, 1 mM CaCl2 for 2 h at 28°C on a rotating shaker (40 rpm). After filtration through a 65-μm nylon mesh, the protoplasts were collected by 3 min centrifugation at 200 g, followed by centrifugation at 100 g in floating solution (25% sucrose (w/v), 10 mM MES, 20 mM KCl). Floating protoplasts were washed in 0.6 M mannitol, 4 mM MES, 20 mM KCl and counted. Electroporation was performed with 2.5 × 105 protoplasts and 40 μg of each plasmid in 300 μl 4 mM MES-KOH, pH 5.7, 0.6 M mannitol, 20 mM KCl. Transformed protoplasts were incubated in the dark at 25°C for 20 h in 4 mM MES-KOH, pH 5.7, 0.6 M mannitol, 4 mM KCl.
Confocal microscope images were taken using an Olympus FV1000 confocal laser microscope with a 40× water objective. The excitation wavelength for eGFP detection was 488 nm.
Isolation of ZmTSA
In maize, four genes encoding TSA homologs are present. Bx1, Igl, and ZmTSAlike have been described previously [17, 18]. A search of the GeneBank and maize genomic database (see Availability and requirements section for URL) revealed putative TSA sequences, which do not constitute alleles of Bx1, Igl, or ZmTSAlike. These sequences, partly represented by the Tentative Unique Gene (TUG) Zmtuc03-08-11.4557, correspond to a new gene, now designated ZmTSA. ZmTSA is located on chromosome 7 (contig AC191027, GeneBank). A full-length ZmTSA cDNA clone was isolated [GenBank:EU334442]. Most plant TSAs have divergent N-terminal sequences that have no counterpart in bacteria and represent transit peptides for plastid import. When this variable part is excluded from the analysis, ZmTSA is 63% identical to BX1, 67% to IGL, and 72% to ZmTSAlike on protein level, respectively [for an alignment, see Additional file 1].
Expression and subcellular localization
For a further characterization of the closely related genes ZmTSA and ZmTSAlike their transcription levels were determined by RT-PCR in different tissues and developmental stages. ZmTSA and ZmTSAlike transcripts were detected in all 16 tissues analyzed [see Additional file 2] and generally, ZmTSA was the predominant isoform expressed. The average transcript levels relative to GAPDH in these preparations were determined as 1.80 ± 0.93 fg fg-1 for ZmTSA and 0.20 ± 0.15 fg fg-1 for ZmTSAlike. The virtually homogeneous expression of ZmTSA and ZmTSAlike qualifies them as candidates to function in tryptophan biosynthesis in maize. In contrast, Bx1 is predominantly transcribed in seedlings and Igl is specifically induced in response to herbivore attack [17, 18, 20].
There is a number of data suggesting that plant tryptophan biosynthesis is predominantly localized in the plastids [13, 29]. ZmTSA and ZmTSAlike were analyzed in silico for putative targeting sequences using "TargetP" and "iPSORT" [22, 23]. ZmTSA was predicted to be plastid localized, while ZmTSAlike, 45 amino acids shorter at the N-terminus, was expected to be retained in the cytoplasm.
ZmTSA has tryptophan synthase α activity
Kinetic parameters of Zea mays tryptophan synthase and comparison with characterized homologs.
458 ± 94
0.006 ± 0.002
52 ± 6
0.019 ± 0.004
24 ± 4
0.29 ± 0.04
28 ± 6
0.44 ± 0.07
47 ± 9
0.45 ± 0.06
EcTSA + EcTSB 1)
StTSA + StTSB 2)
StTSA + StTSB 2)
StTSA + StTSB 2)
Formation of a tryptophan synthase complex in vitro
No interaction of ZmTSB1 with ZmTSAlike, BX1, or IGL was detectable. The native molecular masses of ZmTSAlike, BX1, and IGL were estimated by gel filtration to around 30 kD, indicating that these proteins were monomers in solution (Figure 3B and data not shown). ZmTSB1 apparently formed dimers of approximately 90 kD (Figure 3B,C). No prominent larger complexes were observed. ZmTSAlike remained inactive with IGP as substrate also when the β-subunit was added to the preparation (Table 1). The experiment was repeated with thrombin digested proteins to exclude lack of complexation due to sterical hindrance caused by the His-tag yielding identical results (data not shown).
Tryptophan synthase activity in leaf protein extracts
ZmTSA is a component of the tryptophan synthase complex in maize
To identify constituents of the 160 kD TS complex in planta a mass-spectrometry-based approach was applied. Sequence qualifying peptides, i.e. peptides allowing annotation, were obtained for ZmTSA, ZmTSAlike, BX1, IGL, and ZmTSB1 by the analysis of tryptic digests of recombinant proteins using liquid chromatography with time-of-flight mass spectrometry coupled by electrospray ionisation (LC-ESI-ToF-MS). The resulting detection signals of peptides were compared with theoretically expected tryptic peptide masses [see Additional files 3 and 4]. 46.0% of the total sequence was covered for ZmTSA, 36.5% for ZmTSAlike, 40.4% for ZmTSB1, 50.0% for BX1, and 56.9% for IGL.
To identify the active α-subunit of the TS complex in maize, TS activity was partially purified from leaf extracts by subsequent ion exchange and size exclusion chromatography. The 160 kD protein fraction was concentrated and separated by SDS-PAGE. Proteins between approx. 25 kD (smaller than TSA size) and 60 kD (larger than TSB size) were cut out of the gel. A tryptic digest of these proteins was analyzed by LC-ESI-ToF-MS and surveyed for peptides sequence qualifying for ZmTSA, ZmTSAlike, BX1, IGL, and ZmTSB1.
ZmTSA sequence qualifying peptides identified in a tryptic digest of tryptophan synthase partially purified from leaves.
ret. time (min)
Evidence for physical interaction of tryptophan synthase α and β subunits in plants was provided by immunoaffinity chromatography for Arabidopsis  and by size exclusion chromatography for maize (this study). For the identification of the maize tryptophan synthase components in vivo, specific sequence qualifying peptides were assigned by LC-MS. This approach allows the analysis of enzyme complexes that are not sufficiently stable for application of a larger variety of chromatographic separations, necessary for purification to homogeneity. Applying this method, ZmTSA was identified as α-subunit of a tryptophan synthase complex.
The apparent molecular weight strongly indicates that in maize tryptophan synthase is functional as αββα heterotetramer, similar as in bacteria [30, 31]. In maize ZmTSA is the principal α-subunit of the complex. Catalytic efficiency of ZmTSA was enhanced more than 30-fold by interaction with the β-subunit (Table 1). Such activating interaction between α- and β-subunit is also well known from bacteria like E. coli (Table 1). In E. coli this activation was mutual, i.e. β-activity strongly increased upon formation of an α2β2 TS complex . In contrast, no significant activation of the maize β-subunit by ZmTSA was observed (Table 1). The reason for this difference between the bacterial and plant enzyme is not known.
In maize, ancestral TSAs have been recruited for secondary metabolism. The TSA homologs BX1 and IGL catalyze the formation of indole, which functions as DIMBOA precursor or volatile signal [17–19] (Table 1) and BX1 monomer activity was observed in leaf extracts (Figure 4). In addition, TSB dimers that have been observed in leaf extracts (Figure 4) and in vitro (Figure 3), which possibly function in salvage of indole by its conversion to tryptophan. It remains open, whether a mechanism involving BX1 monomer and TSB dimer contributes significantly to the total metabolic flux towards tryptophan. The bx1 mutant and the bx1 igl double mutant (M. Frey, unpublished data) are fully viable; therefore this process is not essential at any stage of development.
We propose that, despite the availability of the highly active monomers BX1 and IGL, in maize tryptophan is predominantly synthesized through a tryptophan synthase complex containing ZmTSA and ZmTSB1 or ZmTSB2, respectively. Probably this complex has been retained during evolution for tryptophan synthesis, as it enables substrate channelling and allosteric regulation. Knockout mutants could serve as ultimate proof for ZmTSA being essential. Therefore we have extensively screened public databases as well as the large Pioneer HiBred TUSC collection for Mu-transposon insertion mutants  in ZmTSA. However, no insertion alleles were identified. It remains open, whether Zmtsa knockout mutants are lethal.
Based on import studies, subcellular fractionation, and target sequence prediction, it is suggested that in plants the biosynthesis of aromatic amino acids is predominantly localized in plastids (for review, see ). Consistent with these data ZmTSA contains a chloroplast targeting sequence and ZmTSA::GFP fusion proteins were targeted to the plastid (Figure 2). Interestingly, the TSA homolog ZmTSAlike lacks such a transit peptide and ZmTSAlike::GFP fusion proteins were located in the cytosol (Figure 2). It has been debated whether the biosynthesis of aromatic amino acids is also partially active in the cytoplasm, as e.g. a cytoplasmic isoform of chorismate mutase is expressed in Arabidopsis . As recombinant ZmTSAlike, expressed in E. coli, did not show any α-activity it remains unclear, whether ZmTSAlike functions as cytosolic TSA isoform. ZmTSAlike might either require specific conditions and modifications or it has an unknown function in plant metabolism.
Four TSA homologs exist in maize. Only one of these isoforms, ZmTSA, is involved in the formation of a tryptophan synthase complex. Based on our data and previous results for Arabidopsis thaliana we propose that a ubiquitous tryptophan synthase complex is responsible for tryptophan formation in angiosperms, like in fungi and bacteria.
Availability and requirements
Maize genomic database: http://www.maizegdb.org
indole-3-glycerol phosphate lyase
tryptophan synthase alpha subunit
tryptophan synthase beta subunit
weeks after pollination.
We thank U. Genschel for comments on the manuscript and K. Fütterer and S. Grosse for practical assistance. Agilent Technologies is acknowledged for the loan of the HPLC system. This work has been supported by the Deutsche Forschungsgemeinschaft.
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