Figure 5

ATM1 co-localizes with internalized FM4-64 and with endosomal markers in abaxial leaf epidermis cells of N. benthamiana. Fluorescent chimeras were co-expressed by Agrobacterium infiltration. A. GFP-ATM1(IQ-tail) (dots of 980 ± 145 nm in diameter). B. FM4-64. C. Overlay of A and B (1 optic section). D. GFP-ATM1(IQ-tail) (dots of 630 ± 60 nm). E. FYVE-DsRED. F. Overlay of D and E (1 optic section). G. RFP-ATM1(IQ-tail) (dots of 300 ± 100, colored green for ease of demonstration). H. GFP-ARA7 (colored magenta for ease of demonstration). I. Overlay of G and H (1 optic section). J. RFP-ATM1(IQ-tail) (dots of 570 ± 75 nm, colored green for ease of demonstration). K. ARA6-GFP (colored magenta for ease of demonstration). L. Overlay of J. and K (1 optic section). Arrows show co-localization. Scale bars 5 μm. The microscope focus in A-I was in the cytoplasm while the focus in J-L was on the plasma membrane.