Figure 1

Physical mapping of the locations of individual EZ::TN < Not I/KAN-3> transposon insertions in a soybean bacterial artificial chromosome. Individual GS_43D16 clones containing the EZ::TN <NotI/KAN-3> transposon were digested with NotI. Three NotI fragments, I, II and III released from NotI digestion of GS_43D16 are shown in the last lane. Note that fragment III is comprised of the pBeloBAC11 vector sequence. The top panel shows the gel of NotI digested DNA of GS_43D16 clones carrying the transposon in the NotI Fragment II. The middle panel shows the Southern hybridization data of the gel shown in the top panel. The 245 bp probe for Southern analysis was obtained by PCR of the GS_43D16 end that overlaps with GS_18J19, but not GS_99I16. The lower panel shows the distribution of clones carrying the transposon at various regions of the NotI Fragment II. One dot represented one clone containing the transposon at that particular location of the NotI Fragment II.