Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.)

BMC Plant Biology

Table 2 Absolute frequencies of recovery of conserved and variant fragments among the regenerants from tissue culture of immature embryo (E) and anther (A) explants.

	4DH		5DH		8DH		9DH		16DH
AFLP pattern	E	A	E	A	E	A	E	A	E	A	A+E (%)
Not affected by tissue culture
1111	1761	1746	2966	3234	1806	3077	1570	1529	2952	1966	78.3
1100	274	277	193	230	153	261	257	254	455	306	9.2
0011	102	103	190	222	126	214	113	112	197	127	5.2
Affected by tissue culture
0001	4	3	5	30	4	15	0	6	0	0	0.2
0111	102	101	30	42	35	62	52	53	19	17	1.8
0101	8	8	0	35	2	6	0	10	0	0	0.2
0100	25	31	26	70	9	18	1	20	0	1	0.7
1011	57	62	48	62	34	66	29	35	44	26	1.6
1010	12	12	0	3	21	36	5	4	0	0	0.3
1001	2	2	0	0	0	0	0	1	4	1	0.0
1000	90	87	31	42	47	89	15	13	28	14	1.6
1110	11	16	15	10	14	22	0	1	1	5	0.3
1101	24	24	6	4	10	10	18	22	8	9	0.5
0010	0	0	0	0	0	0	0	0	0	0	0
0110	0	0	0	0	0	0	0	0	0	0	0

ISSN: 1471-2229