Figure 4

Amino acid sequences alignment. Amino acid sequences alignment of peptide sequences (N = N-terminal sequences; I = internal sequences by using in-gel trypsin digestion and sequencing), in bold-faced, of spots 1–4 with the published peanut allergen sequences of Ara h4 (AAD47382), Ara h3 (AAC63045), and iso-Ara h3 (ABI17154) (26). Sequences obtained by N-terminal sequencing are shaded in black. The different amino acid residues are colored in red. The amino acid sequences of Ara h3 IgE-binding epitopes [24] are shaded in gray and the critical amino acids to IgE binding are colored in green and underlined.