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Figure 9 | BMC Plant Biology

Figure 9

From: The PTI1-like kinase ZmPti1a from maize (Zea maysL.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte

Figure 9

ZmPti1a mRNA and protein expression in ZmPti1a -RNAi transgenic plants. (A) Northern and (B) Western analysis of ZmPti1a expression in pollen of wild type (H99, A188), T1 heterozygous transgenic (black bar), and PPT-resistant plants lacking the RNAi construct (#10 Ă— WT; WT Ă— #6). ZmPti1a mRNA and protein levels were quantified densitometrically and mRNA and protein levels in A188 were arbitrarily set to 1, respectively. All other values were calculated as multiples of that. Values of ZmPti1a-RNAi transgenic individuals are indicated in bold. Methylene blue staining of ribosomal RNA and Ponceau staining of proteins are given as a control for loading (C) Northern analysis of ZmPti1a expression in pollen of T2 descendants from selfed heterozygous T1 plants. Pollen of plants #2a and #5a contain no detectable levels of ZmPti1 mRNA. Expression of GAPDH was used as a loading control (D) Western analysis of ZmPTI1a protein expression in pollen of T3 siblings from descendants of plants #2a and #5a. ZmPTI1 protein was decreased to undetectable levels in all individuals analyzed. Numbers refer to transgenic lines; lower case letters indicate siblings of the same line.

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BMC Plant Biology

ISSN: 1471-2229

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