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Table 2 Primers used. Sequences of the primers used for cloning of the promoter and gene fragments, for PCR screening of the putative transgenic plants, and for Real Time quantification of transcript levels. For further details, see Methods.

From: Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase

Primer name

Sequence

Use

B33-149 Up

ACATATTTTACTTTTGACTTT

B33 cloning

B33-1950 Dw

GTAGTGTCACACTTACGC

B33 cloning

B33 Up

AGTCGACAATCATTGTTTTATTTTCTCTTTC

B33 cloning

B33 Dw

AGGATCCAAATTTTGTTGGTGCTTTGAG

B33 cloning

Lcy-e 322 Up

AGAGCTCGGCTGTGGTCCTGCTG

Lcy-e cloning

Lcy-e 1085 Dw

AGGATCCTGGCAAAGATCCACCAAC

Lcy-e cloning

Lcy-e 574 Up

TAGGATCATCATCATCAAG

Pcr screening

Nos-test2

CGCGTATTAAATGTATAATTG

Pcr screening

Tub 865 Up

CTGTGAATTTAATCCCCTTG

Pcr screening

Tub 1260 Dw

TGCGCCTGAACATGGCAGT

Pcr screening

Tub Up

CAGACCTGAGGAAATTGGCTG

Real Time assay

Tub Dw

TTCTTGGCATCCCACATTTGT

Real Time assay

Ubi Up

ACAATGTCAAAGCCAAGATCCA

Real Time assay

Ubi Dw

CGGAGACGGAGCACGAGA

Real Time assay

AS-e Up

CCCCAATTTAGCAGACTCCG

Real Time assay

AS-e Dw

CAACAGAAATTATATGATAATCATCGCAA

Real Time assay

Psy1 Up

CGGTCTGCTATTGTTGCTACTCC

Real Time assay

Psy1 Dw

CAGGAACAGGTATGTCTGGCTTC

Real Time assay

Psy2 Up

AGCTTTAGATAGGTGGGAGGCA

Real Time assay

Psy2 Dw

CAAGTCCATACGCATTCCTTCAA

Real Time assay

PDS Up

AGAGACTTTGCATGCCGATTGT

Real Time assay

PDS Dw

AAAGCATCGCCCTCAACTGT

Real Time assay

ZDS Up

TTGCCATGTCAAAGGCCA

Real Time assay

ZDS Dw

ACAGGCACTCCGACCAATTT

Real Time assay

CrtIso Up

TTGGCAGCAGTAGGACGTAAAC

Real Time assay

CrtIso Dw

TCCCTTCCTTTTCATGTGGAA

Real Time assay

Lcy-e Up

GCCAAAATGGATGTGGCAG

Real Time assay

Lcy-e Dw

CAATGTTGCACCAGTAGGATCAG

Real Time assay

Lut1 Up

CGTTCTCCGCCCAAAAAAC

Real Time assay

Lut1 Dw

TTGGCCTAAAGTAAGTGACCTGG

Real Time assay

Lcy-b Up

AATGGGTGGTCCACTTCCAGTA

Real Time assay

Lcy-b Dw

GGATGGATGAACCATGCCAG

Real Time assay

Chy1 Up

CTTGGCCCAAAACCCACTT

Real Time assay

Chy1 Dw

CCTCAAATTGAGGTTTCAGCTTCT

Real Time assay

Chy2 Up

TTTTGCTGTCTCGAAGAAAGCC

Real Time assay

Chy2 Dw

AGCCAACAGGCAGCTAAACTCT

Real Time assay

ZEP Up

TCATGAATGCTGGCTGCATC

Real Time assay

ZEP Dw

TGCTGCAAAGTCATGCGG

Real Time assay