Figure 2

Construction of plasmids for Agrobacterium -mediated transformation. The patatin B33 promoter and a region of the LCY-e transcript were amplified, respectively, from genomic DNA and tuber cDNA using primers inserting appropriate restriction sites at the ends, resequenced, and cloned in the pBI101 plasmid [21]. White triangles indicate the direction of transcription, black triangles the direction of the coding strand. For details see Methods.