Volume 5 Supplement 1

Cell Biology of Nitric Oxide and Cell Death in Plants

Open Access

The FtsH protease is required for the repair of Photosystem II in the cyanbacterium Synechocystis6803 damaged UV-B radiation

  • Otilia Cheregi1Email author,
  • Cosmin Sicora1,
  • Peter B Kos1,
  • Peter J Nixon2 and
  • Imre Vass1
BMC Plant Biology20055(Suppl 1):S8

DOI: 10.1186/1471-2229-5-S1-S8

Published: 31 May 2005

Background

UV-B radiation inhibits the electron transport of the PSII complex by damaging the Mn cluster of water oxidation and leads to the degradation of the D1 subunit of the reaction center complex [1, 2]. In intact cells the inhibited PSII activity can be restored via de novo synthesis of the D1 subunit [3]. The FtsH protease has been shown to play an important role in the turnover of the D1 protein when induced by visible light both in higher plants and in cyanobacteria [4, 5]. However, the role of FtsH in the damage and repair of PSII under UV-B irradiation has not been clarified. Here we studied this question by using a mutant strain of Synechocystis 6803 cyanobacterium, which lacks the slr0228 gene that encodes one of the FtsH homologues.

Materials and methods

Synechocystis sp. PCC 6803 cells were propagated in BG-11 growth medium in a rotary shaker at 30°C under a 5% CO2-enriched atmosphere. The MFtsH mutant was constructed by interrupting the slr0228 gene with a chloramphenicol resistance cassette as described in Ref. [5]. UV-B irradiation was performed in open glass containers in which the cell suspension of 6.5 Mg Chl ml-1 formed a layer of 10 mm height. A Vilbert-Lourmat VL-215M lamp was used as UV-B light source, with maximal emission at 312 nm, in combination with an 0.1 mm cellulose acetate filter (Clarfoil, Courtaluds Chemicals, UK) in order to screen out any UV-C contribution. The UV-B intensity was 4.8 Wm-2 (≈ 13 MEm-2s-1) at the surface of the cell suspension. UV-induced changes in the transcript level of the FtsH homologue genes were detected by quantitative RT PCR. Light-saturated steady-state rate of oxygen evolution was measured using a Hansatech DW2 O2 electrode at a light intensity of 1000 MEm-2s-1 in the presence of 0.5 mM 2,5-dimethyl-p-benzoquinone as electron acceptor.

Results

The cyanobacterium Synechocystis 6803 has four homologues of the membrane bound ATP-dependent FtsH protease encoded by the slr0228, slr1390, slr1604 and sll1463 genes [6]. We have checked the effect of UV-B irradiation on the relative transcript level of these genes by quantitative RT PCR. As shown by the data in Figure 1. all four genes were induced more than 2-fold after 90 min UV-B exposure with the most significant effect seen in the slr0228 and slr1604 transcripts.
Figure 1

UV-B induced expression of the FtsH homologue genes. The transcript levels were detected by quantitative RT PCR for the slr0228 (right hatch), slr1604 (crossed hatch), slr1390 (left hatch), sll1436 (horizontal hatch) genes

In search for the role of the FtsH protease we studied the UV-B induced damage in the oxygen evolving activity of PSII in the MFtsH mutant from which the slr0228 gene had deleted. When compared to the WT, the MFtsH strain showed accelerated loss of oxygen evolution under UV-B exposure and almost complete lack of recovery under visible light (Fig. 2.). The loss of oxygen evolution in the MFtsH strain has practically the same kinetics as seen in the WT in the presence of the protein synthesis inhibitor lincomycin (Fig. 2.).
Figure 2

UV-B induced loss, and recovery of oxygen evolution. The time course of oxygen evolution was followed in the WT (circles) and MFtsH cells (up triangles) during exposure to UV-B light and subsequent recovery in low intensity (40 MEm-2s-1) visible light. The time course of oxygen evolution is also shown in the presence of lincomycin for the WT cells (down triangles)

Conclusion

Our data demonstrate that the FtsH protease is required for the efficient repair of UV-damaged PSII centers in Synechocystis 6803 cells. Considering that restoration of PSII activity requires de novo synthesis of the D1 reaction center protein [3], we conclude that in the MFtsH strain the degradation and synthesis of the D1 protein is blocked.

Declarations

Acknowledgements

This work has been supported in by a grant from the Hungarian Granting Agency OTKA (T034321).

Authors’ Affiliations

(1)
Institute of Plant Biology, Biological Research Center
(2)
Department of Biological Sciences, Imperial College London, Biochemistry Building

References

  1. Vass I, Sass L, Spetea C, Bakou A, Ghanotakis D, Petrouleas V: UV-B induced inhibition of photosystem II electron transport studied by EPR and chlorophyll fluorescence. Impairment of donor and acceptor side components. Biochemistry. 1996, 35: 8964-8973. 10.1021/bi9530595.View ArticlePubMedGoogle Scholar
  2. Friso G, Spetea C, Giacometti GM, Vass I, Barbato R: Degradation of photosystem II reaction center D1-protein induced by UVB irradiation in isolated thylakoids. Identification and characterization of C- and N-terminal breakdown products. Biochim Biophys Acta. 1994, 1184: 78-84.View ArticleGoogle Scholar
  3. Sass L, Spetea C, Motй Z, Nagy F, Vass I: Repair of UV-B induced damage of Photosystem II via de novo synthesis of the D1 and D2 reaction centre subunits in Synechocystis sp. PCC 6803. Photosynth Res. 1997, 54: 55-62. 10.1023/A:1005895924892.View ArticleGoogle Scholar
  4. Bailey S, Thompson E, Nixon PJ, Horton P, Mullineaux CW, Robinson C, et al: A critical role for the Var2 FtsH homologue of Arabidopsis thaliana in the photosystem II repair cycle in vivo. J Biol Chem. 2002, 277: 2006-2011. 10.1074/jbc.M105878200.View ArticlePubMedGoogle Scholar
  5. Silva P, Thompson E, Bailey S, Kruse O, Mullineaux CW, Robinson C, et al: FtsH Is involved in the early stages of repair of photosystem II in Synechocystis sp PCC 6803. Plant Cell. 2003, 15: 2152-2164. 10.1105/tpc.012609.PubMed CentralView ArticlePubMedGoogle Scholar
  6. Sokolenko A, Pojidaeva E, Zinchenko V, Panichkin V, Glaser VM, Herrmann RG, et al: The gene complement for proteolysis in the cyanobacterium Synechocystis sp. PCC 6803 and Arabidopsis thaliana chloroplasts. Curr Genet. 2002, 41: 291-310. 10.1007/s00294-002-0309-8.View ArticlePubMedGoogle Scholar

Copyright

© The Author(s) 2005

Advertisement