Volume 5 Supplement 1

Cell Biology of Nitric Oxide and Cell Death in Plants

Open Access

High-scale analysis of pathogenicity determinants of Potato mop-top virus

  • Andrey Zamyatnin1, 2Email author,
  • Natasha Yelina3,
  • Nina Lukhovitskaya3,
  • Andrey Solovyev3,
  • Anna Germundsson2,
  • Maria Sandgren2,
  • Sergey Morozov3,
  • Jari Valkonen2 and
  • Eugene Savenkov2
BMC Plant Biology20055(Suppl 1):S39

DOI: 10.1186/1471-2229-5-S1-S39

Published: 31 May 2005

Potato mop-top virus (PMTV) is the type member of the genus Pomovirus characterized by a tripartite, single-stranded, positive-sense RNA genome. However, PMTV differs from the other pomoviruses by having an open reading frame (ORF) for a 8-kDa cysteine-rich protein (CRP) on RNA 3, downstream the triple gene block. Site-directed mutagenesis of infectious cDNA clones of PMTV showed that the CRP ORF is not needed for the systemic infection in Nicotiana benthamiana and several other hosts, but it has an effect on the symptom development of PMTV. CRP of PMTV was not capable of RNA silencing suppression in agroinfiltration tests. However, the expression of CRP using vectors based on Potato virus X (PVX) or Tobacco mosaic virus (TMV) resulted in necrotic symptoms on N. benthamiana and tobacco plants, respectively. Sequencing of PMTV isolates obtained from the field revealed significant sequence variability of the CRP. These data suggest that the functions of CRP may be expressed only in certain hosts or at certain phases of the PMTV infection cycle.

Authors’ Affiliations

(1)
Natural Sciences Center of A.M. Prokhorov General Physics Institute, Russian Academy of Sciences
(2)
Department of Plant Biology and Forest Genetics, Genetics Centre, SLU
(3)
A. N. Belozersky Institute of Physico-Chemical Biology and Department of Virology, Moscow State University

Copyright

© The Author(s) 2005

Advertisement