Volume 5 Supplement 1

Cell Biology of Nitric Oxide and Cell Death in Plants

Open Access

Involvement of ethylene, oxidative stress and lipid-derived signals in cadmium-induced programmed cell death in tomato suspension cells

  • Elena Iakimova1Email author,
  • Veneta Kapchina-Toteva2,
  • Anke de Jong3,
  • Atanas Atanassov1 and
  • Ernst Woltering3
BMC Plant Biology20055(Suppl 1):S19

DOI: 10.1186/1471-2229-5-S1-S19

Published: 31 May 2005

Backgraund

Extensive research is ongoing looking for the characterisation of programmed cell death (PCD) in plants involving pathogen attack, chemical elicitation and abiotic inducers, but there are still limited reports on the role of heavy metals in PCD induction and little is known about cadmium-triggered signal transduction in plant systems. Contamination of biosphere with heavy metals has hazardous effect on agricultural crops and human health. In animal models, cadmium intoxication occurs through apoptosis appearing by apoptotic phenotype and an oxidative stress is involved in the mechanism of Cd action. The goal of this present work was to investigate if programmed cell death occurs in cadmium-treated tomato suspension cells; to identify some of the biochemical processes contributing to the signal transduction pathway(s) involved in cadmium toxicity; to investigate the role of oxidative stress (hydrogen peroxide), ethylene and lipid-derived signals and to look for similarities between cadmium- and camptothecin-induced cell death.

Materials and methods

The experiments were undertaken with tomato suspension cells, line Msk8. Specific inhibitors of different biochemical steps were administrated simultaneously with either CdSO4 or topoisomerase-1 inhibitor camptothecin (CPT). Cell viability (FDA staining of the viable cells) was determined after 24 hours and the dynamics of H2O2 production was measured by chemiluminescence in a ferricyanide-catalised oxidation of luminol. Specific caspase peptide inhibitors, antioxidants, NADPH oxidase inhibitors, calcium channel blockers, inhibitors of phospholipid cycle, protein kinase inhibitor and ethylene blockers were tested. Ethylene was applied during 24 h in concentrations up to 100 ppm in the head space. For details on methodology see [1, 2].

Results

The human caspase-1 inhibitor Ac-YVAD-CMK and the broad range caspase inhibitor Z-Asp-CH2-DCB, abolished the cell death of Cd-treated and CPT-treated cells (See Table 1). This strongly suggests that the cell death pathway that is induced by cadmium employs caspase-like proteases and gives a reason to assume that in tomato suspension cells Cd-triggered cell death most probably resembles features of programmed cell death. The amount of hydrogen peroxide increased in response to Cd and CPT. Efficient inhibition of cell death occurred at the application of antioxidants and calcium channel blocker (See Table 1). The inhibition of NADPH oxidase by imidazole, quercetin and kaempferol significantly reduced the percentage of dead cells.
Table 1

Effect of caspase peptide inhibitors, antioxidants (ascorbic acid, catalase, spermine) and calcium channel blocker LaCl3on viability of CPT- or Cd-treated tomato suspension cells

Chemicals

Cell viability (%)

Control

97.5

CPT 5 μM

72.5

CdSO4100 μM

65.0

CPT μM + Ac-YVAD-CMK 100 μM

92.5

CdSO4100 μM + Ac-YVAD-CMK 100 μM

94.0

CdSO4100 μM + Z-asp-CH2-DCB 100 μM

91.5

CPT 5 μM + ascorbic acid 100 μM

93.5

CPT 5 μM + catalase 10 Units/ml

91.5

CPT 5 μM + spermine 100 μM

88.5

CdSO4100 μM + ascorbic acid 100 μM

92.5

CdSO4100 μM + catalase 10 Units/ml

91.5

CdSO4100 μM + spermine 100 μM

88.2

CPT + 5 μM + LaCl3100 μM

95.0

CdSO4100 μM + LaCl3100 μM

94.5

Treatments with ethylene further decreased both Cd- and CPT-reduced cell viability. Comparative experiments with Cd- or CPT-treated cells revealed an analogy in cell response to the ethylene inhibitor AVG (See Table 2). AVG greatly reduced the cell death that was enhanced in response to Cd or CPT. An increase of endogenous ethylene production (measured by laser photoacoustics) occurred in cadmium-treated cells. The data are a clear demonstration of ethylene involvement in Cd- and CPT-triggered cell death. Administration of IP3 cycle inhibitors showed a strong inhibition to Cd-induced cell death.
Table 2

Effect of ethylene and ethylene inhibitor AVG on cell viability of CPT and cadmium treated tomato cell suspension

Chemicals

Cell viability (%)

Control

95

CPT 5 μM

72

CdSO4 100 μM

68

AVG 10 μM

98

Ethylene (Eth) 100 μL/L

95

CPT 5 μM + Eth 100 μL/L

50

CdSO4 100 μM + Eth 100 μL/L

48

CPT 5 μM + AVG 10 μM

90

CdSO4 100 μM + AVG 10 μM

86

CPT 5 μM +AVG 10 μM + Eth 100 μL/L

38

CdSO4 100 μM + AVG 10 μM + Eth 100 μL/L

49

Conclusion

Evidence is accumulating that caspase-like cysteine proteases showing functional similarity to animal caspases, participate in the programmed cell death in plants. In addition to discoveries that caspase-like proteases are involved in cell death in response to pathogen invasion, abiotic stresses and chemical elicitation, our data show that cell death induced by cadmium is also a form of programmed cell death mediated by caspase-like proteases. We have established a key role of hydrogen peroxide and calcium in cadmium-induced apoptotic cell death and have demonstrated that oxidative stress is associated with both cadmium and camptothecin-triggered cell death. We have also shown that polyamine spermine can effectively preserve the cell viability at conditions of chemical stress.

Ethylene was found to be an important mediator of plant cell death. The finding that ethylene greatly stimulated cadmium-induced cell death and that cadmium treatment enhanced endogenous ethylene production indicated that ethylene participates in cadmium-induced cell death in tomato suspension cells. The application of specific inhibitors of phospholipase C, phospholipase D, inositolphosphate monophosphatase, inositol-3-phosphate kinase and phosphatidic acid caused considerable decrease of Cd-stimulated cell death and are the first more detailed evidence that Cd-triggered cell death in plants involves the phospholipid pathway.

Collectively, the cell response to cadmium elicitation and the inhibitors indicate that Cd-triggered cell death is analogous to cell death in response to CPT treatment [14] and involves caspase-like proteases, oxidative stress and ethylene. Cd-induced cell death in plant cells exhibits similarities to HR [5] and cell death induced by known apoptosis inducing chemicals and to its effect in animal systems.

Declarations

Acknowledgements

Elena Iakimova and Veneta Kapchina-Toteva were supported by STSM grants (COST Action 844) and FP5 IHP LSF grants. Authors are thankful to France Harren and Luc-Jan Laarhoven for the assistance with the laser photoacoustics.

Authors’ Affiliations

(1)
AgroBioInstitute
(2)
Faculty of Biology, University of Sofia
(3)
Agrotechnology and Food Innovations, Wageningen University and Research Center

References

  1. de Jong A, Yakimova E, Hoeberichts F, Maximova E, Woltering E: Chemical-induced apoptotic cell death in tomato cells: Involvement of caspase-like proteases. Planta. 2000, 211: 656-662. 10.1007/s004250000341.View ArticlePubMedGoogle Scholar
  2. de Jong A, Yakimova E, Kapchina V, Woltering E: A critical role of ethylene in hydrogen peroxide release during programmed cell death in tomato suspension cells. Planta. 2002, 214: 537-545. 10.1007/s004250100654.View ArticleGoogle Scholar
  3. Woltering E, van der Bent A, Hoeberichts F: Do plant caspases exist?. Plant Physiol. 2002, 130: 1764-1769. 10.1104/pp.006338.PubMed CentralView ArticlePubMedGoogle Scholar
  4. Woltering E, de Jong A, Iakimova E, Kapchina V, Hoeberichts F: Ethylene: Mediator of oxidative stress and programmed cell death in plants. Biology and Biotechnology of the Plant Hormone Ethylene III. Edited by: Vendrell. 2003, IOS Press, 315-323.Google Scholar
  5. Iakimova E, Batchvarova R, Kapchina-Toteva V, Popov T, Atanassov A, Woltering E: Inhibition of apoptotic cell death induced by Pseudomonas syringae pv. tabaci and mycotoxin Fumonisin B1. Biotech &Biotech Eq. 2004:18: 34-46.View ArticleGoogle Scholar

Copyright

© The Author(s) 2005

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