Figure 4

(a) Northern blot detection of vspB transcripts in R₁ transgenic maize. Thirty micrograms of total RNA were separated on 1.2% agarose formaldehyde gels and blotted to Hybond N⁺ membranes. The vspB transcripts were detected by hybridized with a digoxigenin labeled vspB probe. (b) Western blot detection of VSP in R₀ and their progeny (R₁) transgenic maize. Thirty micrograms of protein in extracts from leaves of 7 weeks old plants were separated on 12% SDS-PAGE, blotted onto Hybond-P membrane, and VSP was immunodetected using VSPβ antiserum and the Renascence kit chemiluminescent detection method (NEN Life Sciences Products, Inc).