Table 1 Primers for cDNA cloning of GGPP synthase.
Primers | Sequences (5' to 3') | Designed from |
|---|---|---|
A | GGGGTACCYITTGYATHGCIGCITAYGA | conserved sequence (LCIAACE) |
B | GGGGTACCGTIGARATGATHCAYACIAT | conserved sequence (VEMIHTM) |
C | CGGGATCCTTIGTIACRTCIARDATRTC | conserved sequence (DILDVTK) |
D | CGGGATCCARRTCYTTICCIGCIGTYTT | conserved sequence (KTAGKDL) |
E | GACTCGTCTAGAGGATCCCGT | adapter primer |
F | GACTCGTCTAGAGGATCCCGT17 | oligo-dT adapter primer |
G | GGGGTACCCCTTATGGTTGGTGGGCTTC | degenerated PCR product (KPTNHK) |
H | CGCCACGTTCTCGCCGTAGA | degenerated PCR product (VYGENVA) |
I | CCATATTGGGTGGCGCCGAT | degenerated PCR product (AILGGAD) |
J | GGGGTACCGATGAGGCGGTGGAGAAGCT | degenerated PCR product (DEAVEKL) |
K | ATTTGGTGTTCGACGACGAC | 5'RACE |
L | GGGGTACCCATATGAGCCTCATCGCGAGTC | 5'RACE |
M | GCACGCGTCGACATTGTTCCGGTAAGCAAT | 3'RACE |
N | AGTGAAATGGAAATTATTCA | 3'RACE |
O | GGGGTACCGATGAGCCTCATCGCGAGTCCA | 5'RACE |
P | GCACGCGTCGACAACAATGAGCCTCATCGCG | N-terminal sequence (MSLIAS) |
Q | CATGCCATGGGGTTCACCCGCTCTGCCTTCT | N-terminal sequence (EKAERVN) |
R | TTCTTGGATTTATGAAAGACGAACAAC | 18S rRNA |
S | AAGACCAACAATTGCAATGATCTATCC | 18S rRNA |