Figure 3
Introduction of gus and nptII gene in T. aestivum cv. CPAN1676. A. PCR screening of genomic DNA samples of putative transformants of Triticum aestivum cv CPAN1676 using primers specific to gus gene. The transformants were obtained after bombardment of mature embryo-derived calli with pBI101::Act1. The plasmid pBI101::Act1 and the genomic DNA isolated from untransformed plant were used as the positive and the negative controls, respectively. B. Autoradiograph showing NPTII activity in dot blots of some of the T0 transformants, C. Result of paromomycin leaf spray on T0 transformants showing the functional activity of nptII gene (Extreme left: control)