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Table 3 List of genes and isoenzymes used

From: The molecular genetic linkage map of the model legume Medicago truncatula: an essential tool for comparative legume genomics and the isolation of agronomically important genes

Markers

Nature

Function

Primer sequences (5'-3')

RE

LG

References

ENBP1

SSR

Chloroplastic protein

L: CACTTCCCACTGTCCTAGTCCTAC R: GACTCGTCATCACCAGTTTCATCC

 

1

[65]*

ENOD8

CAPS

Nodulin

L: ATGGTGCAAACTTCGCATCAGGAGG R: ACAACCCTTTGGTCCTGTGCCGTG

SspI

1

[66]

MtPT-1

CAPS

Phosphate transporter

L: TTGCTAAGAACCCGAAACAAGCTGC R: ATGTTCATGATCCTTCTTGCTCAAGC

RsaI

1

[13]

PEPC

CAPS

PhosphoEnolPyruvate Carboxylase

L: CTCATCCTACTCAGTCGGTTCGTCG R: ACACGTAGCTCATCGTTGCAACGCC

RsaI

2

[67]

Z-F1

RFLP

Zn-Finger1

 

EcoRI

3

[68]

GS.B

PCR

Glutamine synthetase

L: AGAAGTAGCGTGGTGGC R: GCTGCCTATGGAGAAGG

 

3

[69]

Mtlec2

SSR

Lectine 2

L: AAAGAATTCCATCTTCCAAGGCGA R: GAAGTCTAGAAACCAATCCTTACCC

 

3

[70]

ENOD12

PCR

Nodulin

L: CCTGCTTATAGGCCACCAC R: CTTCTGCTGGAGGATGCC

 

3

[71]

ATPase

SSR

Vacuolar ATPase

L: GGGTTTTTGATCCAGATCTT R: AAGGTGGTCATACGAGCTCC

 

4

[15]**

MtST-1

PCR

Sucrose transporter

L: ATCAAAGGTGGATGGGGATGGAG R: TAGACCAGAAGGGATGTGATTTCC

 

4

[72]

PGM

Isoenz.

PhosphoGlucoMutase

  

4

[34]

Gs.C

CAPS

Glutamine synthetase

L: GCACAAGGAGCACATTG R: GCCCATAATTAAACATCATG

VspI

5

[69]

Lb I

CAPS

Leghemoglobin I

L: GATAGTCCTCAACTCCAAGC R: CCTGTTGCTCGAAGTTGAGA

SspI

5

[73]

rDNA

CAPS

45s Ribosomal DNA

L: ATGGTCCGGTGAAGTGTTCG R: CCCGGTTCGCTCGCCGTTAC

XbaI

5

This paper

ENOD40

CAPS

Nodulin

L: TTAGTAGGATCTTCTCTTTCACTAGC R: GCCTCCGATTATCAAAGGTCAAG

AluI

5

[74]

GS.A

PCR

Glutamine synthetase

L: AAAGACAAGTGGTTGTGAC R: GCTGCCTATGGAGAAGG

 

6

[69]

MtN1

PCR

Nodulin

L: ATCTATCTTCTTCGCTC R: TGACTGTTGAACATCTC

 

6

[75]

GSHS

SSR

Glutathione synthetase

L: AGCAATAGGCAATGGCTGCTCCTGC R: AATGGTGAGATGCTTATGATGAGAC

 

7

[76]

VR

CAPS

Vestitone reductase

L: GAGTGTGTGTAACTGGAGGTACAGG R: ATGCCTAATGCGCCATCGACGGTTC

DdeI

7

[77]

PGD

Isoenz.

PhosphoGlucoDeshydrogenase

  

7

[34]

ENOD16

RFLP

Nodulin

L: ATCGGGACACTCTTTAGCAAGACCC R: TGGGCGGTGGAATTGGACCTCTTG

EcoRV

8

[78]

  1. Underlined markers have been used for synteny studies RE : Restriction Enzyme LG : M. truncatula Linkage Group SSR : Simple Sequence Repeat: polymorphism is revealed by the length of the amplified fragment CAPS : Cleaved Amplified Polymorphic Sequence : polymorphism is revealed after digestion of the amplified fragment with restriction enzymes PCR : Polymerase Chain Reaction : polymorphism is revealed by the length of the amplified fragment L : Left primer R : Right primer *: Accession n° AJ002479 ** : Accession n° AA660456
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BMC Plant Biology

ISSN: 1471-2229

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