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Table 2 Segregation of T 1 plants transformed with pHEX22 (SP-NaD1ΔCTPP)

From: The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions

Line

No. plants

PCR -ve

PCR + ve

Homozygous

Hemizygous

78.131.1

45

8 (18%)

37 (82%)

14 (31%)

23 (51%)

83.102.1

36

10 (28%)

26 (72%)

8 (22%)

18 (50%)

83.68.1

29

2 (7%)

27 (93%)

MC

MC

83.23.2

21

1 (5%)

20 (95%)

MC

MC

  1. The presence of NaD1 was determined by PCR. Lines 73.131 and 83.102 plants were identified as homozygous or hemizygous by real time PCR. Values in parentheses indicate the percentage of the tested plants. Lines 83.68.1 and 83.23.2 had more than one DNA insertion so zygosity was not determined. MC = multicopy lines.
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BMC Plant Biology

ISSN: 1471-2229

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