Figure 6
Location of GFP in N. benthamiana cells after transient expression of various GFP-chimera constructs. Micrographs showing the location of GFP produced during transient expression of (A) SP-GFP-CTPPNaD1, (B) SP-GFP-VSDNaPI, (C) SP-GFP or (D) GFP constructs in the leaves of N. benthamiana. The left panels show GFP fluorescence, the middle panels reveal fluorescence from the FM5-95 dye and the right panels are merge images (c, cytoplasm; ec, extracellular; pm, plasma membrane; t, tonoplast; v, vacuole).