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Figure 2 | BMC Plant Biology

Figure 2

From: Exploring mechanisms linked to differentiation and function of dimorphic chloroplasts in the single cell C4 species Bienertia sinuspersici

Figure 2

Confocal images of GFP expression in Bienertia protoplasts from the construct RbcS-FL spGFP (A-C) and the construct RbcS-FL roGFP2 (D-F). The roGFP2 protein is redox sensitive, while the spGFP protein is redox insensitive. Cells were scanned for a focal plane closest to the slide surface to maximize GFP fluorescence from each chloroplast type. Images A and D are GFP emission. Images B and E are chlorophyll autofluorescence. Images C and F are the merged images of GFP and chlorophyll autofluorescence emission. CCC = central compartment chloroplast, PCC = peripheral compartment chloroplast. Scale Bar = 50 μm.

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