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Figure 2 | BMC Plant Biology

Figure 2

From: Deletions of the SACPD-C locus elevate seed stearic acid levels but also result in fatty acid and morphological alterations in nitrogen fixing nodules

Figure 2

Comparative Genome Hybridization analysis of MM106 in comparison to ‘Bay’. (a) Entire genome view of Comparative Genome Hybridization for MM106/’Bay’. Deletion affecting Gm14 is indicated by arrow. (b) PCR assay for detecting stearoyl-acyl carrier protein desaturase (SACPD) gene deletions from ‘Bay’ radiation induced mutant lines. L indicate molecular weight ladder, “-“ indicates negative control. (c) Southern blot analysis with a SACPD-C specific probe against DNA from MM106 (1), ‘Williams 82’(2), and FN8 F2:3 segregants which displayed typical levels of seed stearic acid (4–6) and FN8 F2:3 samples which displayed elevated levels of seed stearic acid (7–9).

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BMC Plant Biology

ISSN: 1471-2229

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