Perturbing the metabolic dynamics of myo-inositol in developing Brassica napus seeds through in vivo methylation impacts its utilization as phytate precursor and affects downstream metabolic pathways

Table 1 Modified translational contexts for IMT gene driven by the phaseolin promoter

Name of construct	Line	Sequence
pPhIMT1 (parent-transgenic IMT)	Ph1-18	A^-3A^-2A^-1 ATG A
pPhIMT2	Ph2-15	G^-3C^-2C^-1 ATG A
pPhIMT3	Ph3-19	A^-3C^-2C^-1 ATG A

Sequence column shows DNA triplets 5' upstream from the translation initiation codon: line Ph1-18 (parent IMT) with dA nucleotides in positions -1 to -3; line Ph2-15 with dC nucleotides in positions -1 and -2 and dG nucleotide in position -3; line Ph3-19 with dC nucleotides in positions -1 and -2 and dA nucleotide in position -3.

ISSN: 1471-2229