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Table 2 Discrimination between hemizygous and homozygous plants

From: Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay

Sample

Rint4-9/EC A

NOS/EC A

Rint4-9/EC ABD

NOS/EC ABD

EC A/EC ABD

HEM1

0.8

0.8

0.8

0.7

1.9

HEM2

0.9

0.9

1.0

0.9

2.1

HEM3

0.9

1.0

1.0

0.9

2.1

HEM4

1.1

1.0

1.2

0.9

2.0

HOM5

2.3

2.1

2.6

2.1

2.0

HOM6

1.9

2.0

2.0

1.9

2.0

HOM7

1.6

2.2

1.8

2.0

2.0

HOM8

1.8

1.7

2.2

1.8

2.3

  1. Headings indicate target regions used in ΔCT analyses, numbers refer to # copies. All comparisons gave similar results (assuming 2 copies for EC A and 6 copies for EC ABD), showing that samples 1–4 were hemizygous and 5–8 were homozygous. The last column shows the number of copies estimated for EC A using EC ABD as the reference.
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BMC Plant Biology

ISSN: 1471-2229

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