Figure 2

Histochemical localization of AtNAG promoter- GUS expression in Arabidopsis . In Arabidopsis transgenic plants carrying the Arabidopsis NAG (AtNAG) promoter- GUS fusion gene, GUS staining is shown in the following tissues: shoot of a seedling at 4 days after germination (DAG) (A); shoot apex and leaf primordia at 4 DAG (B) and 7 DAG (C); vascular bundles in the cotyledon (D) and the hypocotyl (E) at 4 DAG; vasculature (F and G) and stomata (F and H) of a true leaf at 7 DAG; hypocotyl-root junction at 4 DAG (I); root vasculature and emerging lateral root (J); flower buds and axillary buds in the reproductive stage (K), and enlarged pictures of the flower buds (L) and the axillary buds (M); a seedling at 2 DAG upon tunicamycin (TM) treatment at 0 (N), 0.25 (O), and 0.5 μg/ml (P). Scale bars: A = 2 mm, B, C, I, and L = 0.5 mm, D, G, and N-P = 200 μm, E and J = 100 μm, F and M = 1 mm, H = 20 μm, K = 1 cm.