SMART – Sunflower Mutant population And Reverse genetic Tool for crop improvement

BMC Plant Biology

Table 3 Types of induced mutation in FatA and SAD and mutation frequency

Target genes	Function	Fragment Size (bp)	Identified mutations						Mutation frequency (1/kb)
Target genes	Function	Fragment Size (bp)	Missense	Stop codon	Silent	Intronic	Promoter region	Total	Mutation frequency (1/kb)
FatA	Fatty acid biosynthesis	1,881	3	1	6	1	2	12	1/783
SAD	Fatty acid biosynthesis	619	6	0	8	0	0	14	1/221
Total		2,500	9	1	13	1	2	26	1/480

The mutation frequency for each amplicon is calculated as follows:
[(size of the amplicon) × (total number of samples screened)] / (total number of identified mutants). The average mutation frequency was estimated to be one mutation per 480 kb.

ISSN: 1471-2229