Figure 3

Expression of PvTIFY genes upon JA elicitation. (A) n-Fold expression of PvTIFY genes from different subfamilies in common bean roots incubated with Me-JA. (B–C) Normalized n-fold expression of the PvTIFY10C gene in wild type (B) and transgenic (C) bean roots without Me-JA (time 0, control media; dark blue) or incubated with Me-JA for 30 min (blue). After incubation, Me-JA was depleted from the nutrient solution and 12 h later gene expression was determined (turquoise). Values are normalized to the value from the control conditions (without Me-JA), which was set to 1. Transgenic roots derived from composite bean plants transformed with pPvTIFY-RNAi or pPvTIFY-OE are indicated. Values represent the average of three biological replicates. Asterisks or different letters represent significantly different means according to statistical analysis (p < 0.05).