Vernalization treatment induces site-specific DNA hypermethylation at the VERNALIZATION-A1 (VRN-A1) locus in hexaploid winter wheat

Table 3 Description of amplicons characteristics used for restriction enzyme analyses

Fragment number	Primer name	Sequence (5′-3′)	Position of fragment within theVRN-A1gene (bp from start of Genbank accession AY747600)	Size of amplified fragment (bp)	Number of restriction site within amplicon for:
					MspI	PspGI	BstUI
0.0 k	VRN_1_A_pr_F*	GAAAGGAAAAATTCTGCTCG	44–527	483	8	1 (n.a.)	3
0.0 k	VRN_1_ex1_R*	TGCACCTTCCCSCGCCCCAT	44–527	483	8	1 (n.a.)	3
1.6 k	1.6 k-F	GCCTCCACGGTTTGAAAGTA	1695–2358	663	1	0	2
1.6 k	1.6 k-R	ATCTCAAGATTTTAGTTCCGATCCT	1695–2358	663	1	0	2
3.0 k	3.0 k-F	TGCTGCAGTGATATTTTGTTAGC	3030–3710	680	0	1	0
3.0 k	3.0 k-R	TGATGGGTCATAAGGTTTTGC	3030–3710	680	0	1	0
4.0 k	4.0 k-F	CTTCCTTGGTGGGCTGTG	4003–4607	604	1	0	1
4.0 k	4.0 k-R	TGGCTCTCCACCACAATACC	4003–4607	604	1	0	1
5.0 k	5.0 k-F	GGCTAAGATCGTGAGGAAGG	5014–5648	634	0	1	0
5.0 k	5.0 k-R	TGACTAGCACCACATCAATCG	5014–5648	634	0	1	0
6.8 k (b)	6.8 k (b)-F	GCGGCATCATCTTCTTGC	6834–7148	314	1	0	2
6.8 k (b)	6.8 k (b)-R	GGCTACTCCTCCACCTTATGC	6834–7148	314	1	0	2
9.8 k	9.8 k-F	TCTCCAGTCCTTCGGATTGT	9871–10489	618	1	1	0
9.8 k	9.8 k-R	GGCTTTTGGGTTTCATCTCC	9871–10489	618	1	1	0
11.0 k	11.0 k-F	AATGATTTGATACAGCAGCACAATA	11028–11710	682	1	1	0
	11.0 k-R	ACCAATTCAAAAGATGGTTACTTGA

ISSN: 1471-2229