Figure 8

Time course analysis of TIA metabolite levels. Levels of vindoline, vinblastine and vincristine were below detection thresholds. Unk54 represents a tabersonine-like compound. Results shown are the: uninduced ORCA2-OE line (□, solid line), 0.02% ethanol-induced ORCA2-OE line (■, solid line), uninduced control line (∆, dashed line) and 0.02% ethanol-induced control line (▲, dashed line). Levels of the following metabolites are presented: A) tabersonine, B) ajmalicine, C) lochnericine, D) strictosidine, E) hörhammericine, F) catharanthine, G) serpentine, H) 19OHTab, I) 16OHTab and J) Unk54. Results are the average values of three biological replicates. Error bars indicate standard deviations. Levels of the indicated metabolite in the induced versus uninduced ORCA2-OE cultures differed at the same time point with: * = p ≤ 0.1, ** = p ≤ 0.05 according to Student’s t-test. Student’s t-test results for the induced versus uninduced cultures of the control line are not depicted.