Figure 2

Schematic of binSRNA-ORCA2 overexpression construct used for C. roseus transformation. The CaMV 35S promoter directs transcription of the alcR gene to produce the regulatory protein, AlcR. In the presence of its coinducer, AlcR binds to specific regions within the alcA promoter region and induces transcription of downstream genes [34]. As plants produce the AlcR coinducer, acetaldehyde [35], from ethanol, this construct thus allows ethanol-inducible expression of ORCA2. The npt11 gene codes for kanamycin resistance. The transcription termination sequence from the nos gene is present downstream of ORCA2. alcA, alcA promoter sequence; alcR, alcR coding region; CaMV 35S, CaMV 35S promoter sequence; LB, T-DNA left border sequence; nos, nopaline synthase transcription termination sequence; ORCA2, ORCA2 cDNA coding region; RB, T-DNA right border sequence.