Identification of mildew resistance in wild and cultivated Central Asian grape germplasm

Riaz, Summaira; Boursiquot, Jean-Michel; Dangl, Gerald S; Lacombe, Thierry; Laucou, Valerie; Tenscher, Alan C; Walker, M Andrew

doi:10.1186/1471-2229-13-149

BMC Plant Biology

Table 4 Determination of flower phenotype with a combination of two markers (APT3 and VVIb23) for the V. vinifera subsp. sylvestris used in this study

From: Identification of mildew resistance in wild and cultivated Central Asian grape germplasm

Accession ID	Accession name	Sub	Country/Source	Flower	Flower	APTa	APTb	APTc	APTd	VVIb23
		species	collection	phenotype	genotype
DVIT3348.17	sylvestris	sylvestris	Georgia	H	HF	266	336	397	466	282-286
DVIT3349.08	sylvestris	"	"	M	MF	266	336	397	466	280-286
DVIT3349.12	sylvestris	"	"	H	HF	266	397	466		282-286
DVIT3350.02	sylvestris	"	"	H	HF	266	336	397	466	282-286
DVIT3350.25	sylvestris	"	"	M	MF	266	336	397	466	278-286
DVIT3357.04	sylvestris	"	"	F	FF	266	397			286-286
DVIT3357.30	sylvestris	"	"	H	HF	266	397	466		282-286
DVIT3351.23	sylvestris	"	Armenia	M	MF or MH	266	336	466		294-302
DVIT3351.27	*sylvestris*	"	"	M	MF or MH	336	466			294-302
DVIT3353.31	sylvestris	"	"	F	FF	266	397			288-299
DVIT3353.43	sylvestris	"	"	M	MF	266	336	397	466	290-294
DVIT3355.04	sylvestris	"	"	M	MF	266	336	397	466	294-299
DVIT3355.05	sylvestris	"	"	H	HF	266	336	397	466	282-294
DVIT3356.33	sylvestris	"	"	F	FF	266	336	397		288-299
DVIT3356.38	sylvestris	"	"	M	FF	266	336	397	466	294-299
DVIT1799	O30-51	"	Afghanistan	M	MF or MH	266	397	466		303-322
DVIT1800	O30-53	"	"	H	HF	266	397	466		282-322
DVIT1798	O30-44	"	Iran	M	MF or MH	466	466			303-322
DVIT1802	O33-60	"	"	H	HF	266	397			282-288
DVIT1803	O34-16	"	"	F	FF	266	397			288-303
DVIT1805	O34-26	"	"	M	MF or MH	266	336	397	466	288-299
DVIT1804	O34-29	"	"	H	HF	266	397			282-282
DVIT1806	O34-54	"	"	M	MF or MH	266	397	466		299-302
DVIT1807	O34-55	"	"	M	MF or MH	266	336	397	466	288-288
DVIT1808	O35-07	"	"	F	FF	266	336	397		288-299
DVIT1809	O35-11	"	"	M	MF or MH	266	397	466		299-302
DVIT1811	O35-41	"	"	F	FF	266	397			286-299
DVIT1812	O35-47	"	"	M	MF or MH	266	397	466		286-288
DVIT1813	O35-50	"	"	H	HF	266	397	466		282-286
DVIT1816	O35-64	"	"	H	HF	266	466			282-284
DVIT2442.06	Arybata	"	Turkmenistan	M	MF	266	466			288-307
DVIT2442.01	Arybata	"	"	F	FF	266	397			288-288
DVIT2440.01	Ayedere	"	"	M	MF or MH	266	397	466		299-307
DVIT2440.09	Ayedere	"	"	M	MF or MH	266	397	466		288-307
DVIT2447.09	Uzuntakoy	"	"	H	HF	266	336			282-288
DVIT2447.04	Uzuntakoy	"	"	H	HF	266	336	397	466	282-288
DVIT2445.12	Kara kaytak	"	"	F	FF	266	397			288-288
DVIT2446.12	Yuvankala	"	"	M	MF or MH	266	397	466		288-302
DVIT2446.09	Yuvankala	"	"	H	HF	266	397	466		282-282
DVIT2444.19	Kochtemyr	"	"	H	HF	466	466			282-282
1186Mtp1	Chirai obak	sativa	Tajikistan	H	HF	266	266			282-299
DVIT0576	Husseine	"	Afghanistan	F	FF	266	397			288-288
20008-14 B	Kismish vatkana	"	Uzbekistan	H	HF	266	397	466		282-299
DVIT2323	Karadzhandal	"	Uzbekistan	H	HF	266	397	466		282-299
DVIT0431	Khalchili	"	Afghanistan	H	HF	266	397	466		282-288
ARM Q01-16	Late vavilov	"	Turkmenistan	H	HF	266	397	466		282-288
2642Mtp2	Matrassa	"	Azerbaijan	H	HF	266	397	466		282-288
2657Mtp1	Soïaki	"	Uzbekistan	F	FF	266	266			288-307
DVIT1126	Sochal	"	USSR	F	FF	266	397			288-299
2510Mtp1	Vassarga tchernaia	"	Uzbekistan	F	FF	266	266			288-299

The APT3 marker is capable of distinguishing female-flowered vines from males or hermaphrodites. The second marker, VVIb23 has a unique allele linked to the hermaphrodism. With the combination of these markers, flower phenotypes could be reliably determined (M - male, H – hermaphorditic, F - female). Flower phenotypes of the 10 powdery mildew resistant subsp. sativa are also detailed with these two markers. Powdery mildew resistant genotypes are in bold font.

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ISSN: 1471-2229

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