Figure 3
From: Calmodulin-dependent and calmodulin-independent glutamate decarboxylases in apple fruit
Impact of pH and Ca2+/calmodulin on the activity of various recombinant apple and Arabidopsis GADs. Each panel (A-C) represents a separate experiment and shows results from recombinant proteins prepared simultaneously. The data represent the mean of three technical replicates for each treatment. All proteins, except those tagged with a 6-His motif (designated with the prefix H), were purified by calmodulin-affinity chromatography. While the activity of MdGAD1 was detectable at pH 7.3, with the scale chosen for presentation, the low activity is not evident in the figure. SDS-PAGE analysis of the expression and purification of these proteins is given in Additional file 2: Figure S2.