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Table 1 Chl-a fluorescence parameters as well as pigment and protein contents of the adg1-1/tpt-2 double mutant compared to the wild type (Col-0) and the adg1-1 and tpt-1 single mutants

From: Defects in leaf carbohydrate metabolism compromise acclimation to high light and lead to a high chlorophyll fluorescence phenotype in Arabidopsis thaliana

Lines

Fv/Fmratio

ΦPSII

Chl content (mg·m-2)

Chl a/bratio

Carotenoid content (mg·m-2)

Protein content (g·m-2)

A Plants grown in LL

Col-0

0.744 ± 0.011

0.584 ± 0.016 b, d

127.2 ± 7.5

2.68 ± 0.03 d

4.4 ± 0.3

0.59 ± 0.10

adg1-1

0.751 ± 0.010

0.487 ± 0.077 a, c

133.8 ± 16.6

2.64 ± 0.06 d

4.5 ± 0.6

0.57 ± 0.14

tpt-2

0.753 ± 0.019

0.582 ± 0.030 b, d

135.8 ± 11.3

2.61 ± 0.08 d

4.5 ± 0.3

0.44 ± 0.04

adg1-1/tpt-2

0.746 ± 0.005

0.472 ± 0.022 a, c

135.3 ± 9.2

2.35 ± 0.07 a, b, c

4.7 ± 0.4

0.54 ± 0.05

B Plants grown in HL

Col-0

0.800 ± 0.007 c, d

0.396 ± 0.014 d

286.2 ± 26.5 b, c, d

3.09 ± 0.13 d

10.6 ± 0.5 b, d

3.38 ± 0.42 b, d

adg1-1

0.778 ± 0.007 d

0.366 ± 0.014 d

320.6 ± 24.3a, c, d

3.00 ± 0.04 c, d

12.4 ± 0.9 a, c, d

4.29 ± 0.64 a, c, d

tpt-2

0.757 ± 0.030 a, d

0.359 ± 0.046 d

258.7 ± 9.9a, b, d

3.11 ± 0.07 b, d

10.1 ± 0.6 b, d

3.24 ± 0.42 b, d

adg1-1/tpt-2

0.226 ± 0.021 a, b, c

0.056 ± 0.027 a, b, c

103.0 ± 10.8a, b, c

2.13 ± 0.04 a, b, c

5.5 ± 0.5 a, b, c

2.26 ± 0.48 a, b, c

  1. The plants were grown for 4 weeks either in LL (A) or HL (B). Photosynthesis parameters were determined with the Imaging-PAM fluorometer using the integrated light source. Before the determination of Fv/Fm ratios the plants were dark-adapted for 15 min. The ΦPSII values refer to steady state photosynthesis, which was attained 7-10 min after onset of illumination with PDFs similar to those the plants experienced during growth (i.e. at 30 μmolm-2·s-1 [LL] and 300 μmol·m -2·s -1 [HL], respectively). The data represent the mean ± SE of n = 3 (Fv/Fm ratio, ΦPSII) or n = 5 (Chl content, Chl a/b ratio, carotenoid- and protein content) measurements. Significant differences in the data pairs were identified by the ANOVA test and further analyzed by the Tukey-Kramer test. The letters as superscripts denote the lines to which a significant difference (P < 0.05) was found with a = Col-0, b = adg1-1, c = tpt-2 and d = adg1-1/tpt-2.