Figure 4

Zein accumulation patterns of Hi-Met transgenic seeds. A, Hi-Met transgenic seeds in lane 2 and 4 accumulate significantly higher amounts of the 10-kDa δ-zein, but their β- and γ-zeins are lower than non-transgenic controls in lanes 1 and 4. B, linkage analysis of the Hi-Met transgene and the suppression of β- and γ-zeins in immature seeds from Hi-Met/- x B73 at 18 DAP. The kernels inheriting the Hi-Met transgene expressing higher level of the 10-kDa δ-zein are marked with “T.” C, as described in B, but seeds are at maturity. D, linkage analysis of the Hi-Met transgene and the suppression of β- and γ-zeins in the progeny from the cross of (Met/-; z1CRNAi/-) x (Hi-II B x A). The kernels inheriting the Hi-Met transgene expressing higher level of the 10-kDa δ-zein are marked red. M, protein markers from top to bottom being 37, 25, 20, 15 and 10 kDa. The size of each zein band is indicated with numbers in the “kDa” column.