Figure 3From: Chloroplastic Hsp100 chaperones ClpC2 and ClpD interact in vitro with a transit peptide only when it is located at the N-terminus of a proteinLimited proteolysis assays. GST-TP-FNR was digested with thermolysin. At different time points (see Materials and Methods), aliquots were taken and analyzed by SDS-PAGE stained with Coomassie Brilliant Blue (A), followed by immublotting revealed with antibodies raised against GST (B) or FNR (C). Lanes 1 show the migration of molecular weight standards.Back to article page