Figure 1

Expression patterns of P-INO promoter constructs in wild-type plants. DIC (A O) and light microscopy images of ovules and flowers from plants containing pRJM77 (P-INO::GUS) (A D), pLAW04 (POSX 3′delPOSY to −756::GUS) (E H), pLAW138 (POSX 5′delPOSY to −868::GUS) (I L), pKLP63 (4XPOS6::GUS) (M P), pKLP7 (35Senh:P-INO::GUS) (Q), pKLP4 (35Senh:POSXPOSY::GUS) (R), pKLP6 (35Senh:POSX::GUS) (S) and pKLP26 (35Senh::35SMP::GUS) (T), assayed for GUS activity. Panels (I L) were stained for GUS activity using a 50X elevated concentration of 5-bromo-4-chloro-3-indolyl β-d-glucuronic acid (X-Gluc) relative to panels (A H and M T), to detect GUS activity with intensity and pattern similar to that observed for the full-length promoter. Stages of ovule development: 2-III (A, I, M); 2-IV (E); 2-V (F, J, U); 3-I (B, N, W); 3-III (C, G, K, Y); 4-III (D, H, L, O, AA) (stages according to Schneitz et al. [14]). Confocal microscopy with the constructs P-AtINO::AtINO:GFP [11] (U-X) and P-BoINO::AtINO:GFP (Y-BB), in Arabidopsis. (U, W, Y and AA) show a DIC image overlaid with the green confocal signal from the GFP, or (V, X, Z and BB) show GFP florescence alone. Scale bar: (F) 15 μm, (A, B, E, I, J, M, U, V) 25 μm, (G, N, W, X) 30 μm, (C, K, O) 40 μm, (D, H, L, Y, Z) 50 μm, (P, Q, R, S, T, AA, BB) 700 μm. f, funiculus; o, outer integument; i, inner integument; n, nucellus.