Skip to main content

Table 4 Relative CKX activity in developing and fully developed leaves and 7 DAP spikes in T 1 lines of Golden Promise and one Scarlett line (S) silenced via biolistic and Agrobacterium -mediated methods

From: HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes

Biolistic method

 

Developing leaf

Developed leaf

Spike 7 DAP

Line*

Range

Mean

Range

Mean

Range

Mean

4

1.35 – 2.59

1.89 ±0.41

1.06 – 2.29

1.54 ±0.49

0.65 – 1.21

1.02 ±0.23

5**

0.78 – 4.84

2.55 ±1.57

1.01 – 14.11

3.44 ±3.83

0.40 – 1.13

0.75 ±0.28

6**

0.28 – 7.76

3.22 ±2.86

0.61 – 11.23

3.76 ±3.66

0.80 – 1.90

1.27 ±0.40

7

0.51 – 0.85

0.66 ±0.13

0.46 – 0.76

0.62 ±0.10

1.10 – 1.54

1.25 ±0.15

8

lack of germination

17 S

0.55 – 1.78

1.01 ±0.44

0.12 – 1.28

0.89 ±0.49

0.58 – 1.13

0.70 ±0.22

Agrobacterium -mediated method

 

Developing leaf

Developed leaf

Spike 7 DAP

Line

Range

Mean

Range

Mean

Range

Mean

411

0.64 - 1.09

0.86 ±0.19

0.90 - 1.54

1.14 ±0.26

0.51 - 0.97

0.76 ±0.19

413

0.28 - 1.04

0.74 ±0.26

0.44 - 1.14

0.80 ±0.24

0.69 - 1.12

0.93 ±0.16

415**

0.39 – 1.13

0.70 ±0.25

0.40 – 1.19

0.76 ±0.27

0.40 – 1.33

0.90 ±0.28

426

0.23 – 1.05

0.60 ±0.34

0.34 – 0.97

0.61 ±0.25

0.58 – 1.13

0.87 ±0.23

431**

0.19 – 1.08

0.66 ±0.23

0.39 – 1.54

0.88 ±0.28

0.31 – 1.44

0.90 ±0.27

443

0.35 - 0.96

0.67 ±0.22

0.42 - 1.39

0.77 ±0.36

0.49 - 1.32

0.81 ±0.33

444

0.60 - 1.21

0.90 ±0.25

0.21 - 1.08

0.56 ±0.32

0.91 - 1.18

1.03 ±0.12

  1. * – 5 to 6 plants tested in each line.
  2. ** – 10 to 12 plants tested.
  3. S – Scarlett line.
  4. Lack of significant differences (P<0,05).