Table 2 Primer sets for B-, C-, D- and γ-hordein transcripts and reference genes
Name | Primer sequence forward | Primer sequence reverse |
|---|---|---|
Common B | TTTCCAACAACCTCAACCACA | GTAGGGTACGCAGCGCAAT |
B1 | GCAAGAACAACCCCAACAGTC | GTAGGGTACGCAGCGCAAT |
B1a | CCAGCAACTGCCGCAAATCT | CAACTGTTGTTGGGTTTGGGAT |
DQ148297 | CCACTCCAGCTAGCTCAC | TGCCGAATGGAAGTATGCG |
X87232 | GGGCTACAACAACCAATTCTG | CCTTGTGGGAGTGGTGTTG |
JQ867084 | CCAACAGTCGGTCCAAGGA | CATTGTCCAACTTTCTCCTGC |
B2 | CCGCAGCAAGTCGGACAA | CCTGTTGTTGTTGACCAAC |
B3 | GTGCAATCGTCTACTCTATCG | GGGAGACACCTTGGACCAAT |
B3a | CTATCGTCCTGCGAGAACAAT | CCGACTTGTTGCTGTTGTG |
B3b | ACGTATTGCAAGGTCGCAG | CGGCAGTTGTTGGCAACAC |
B3c | AAACAACAGCAAGTGCCACAT | TCGCCTCAAGCTGAGCTAG |
DQ826387 | CCTCAACCACAACAAGTTGGC | CTGTACGACGGCACATTAACAC |
GQ342976 | CTCCTACAAGAACAACAAGAC | CCTTGCATGGGTTTAGCTGC |
JQ867088 | AACAACAGTCGCAGCTACAT | TTCAAGCTGAGCTAGCTGGA |
Common C | TAATTCCCCAGCAACCTCAA | CCATACTCCAGATGGTTTGTTG |
C1 | TCAACCAGTCCCCCAGCA | CTTGTTGGGGTTGCGGTT |
S66938 | CCTCAACAACCATTTCCCCT | AAATGGTTGTTGTGGTTGCCA |
Common D | CACCGTGTCTCTGCACCATG | TGCCGTAGTACAACTCGTTGG |
Common γ1 | CAACCGCAACAACTAGCTCA | CACCAACAAATGGTGCTTTG |
AJ580585 | CCAACAACAACTGAATCCGTA | TTGCAGGCAACATTGTTGCA |
X13508 | CCTGTGTCATTGTTATCGTACA | CGACAACTGCTCTGTTGCAC |
Common γ3 | GGTTGGGTCATTGGTGATTC | AGCAATAAGGTGGGACATGC |
X72628 | AGCAAATATCAATGAGCAG | GAGATTGGACAAAACCATGAC |
JQ867080 | AGCAAATATCAATGAGCAA | GAGATTGGACAAAACCATGAT |
Actin | CCTCAGTTGAGAAGAGCTACG | TCTGCGCCAATCGTGATC |
Ubiquitin | TCAAGGTGAAGACACTTACTGG | CATAGATGAGCCTCTGTTGAAC |
PTF | CTATGTGCATGTGCGTGTC | CTTGAGAATCTTGTTGTAGCTG |