Histone acetyltransferases in rice (Oryza sativaL.): phylogenetic analysis, subcellular localization and expression
© Liu et al.; licensee BioMed Central Ltd. 2012
Received: 23 November 2011
Accepted: 10 August 2012
Published: 15 August 2012
Histone acetyltransferases (HATs) play an important role in eukaryotic transcription. Eight HATs identified in rice (OsHATs) can be organized into four families, namely the CBP (OsHAC701, OsHAC703, and OsHAC704), TAFII250 (OsHAF701), GNAT (OsHAG702, OsHAG703, and OsHAG704), and MYST (OsHAM701) families. The biological functions of HATs in rice remain unknown, so a comprehensive protein sequence analysis of the HAT families was conducted to investigate their potential functions. In addition, the subcellular localization and expression patterns of the eight OsHATs were analyzed.
On the basis of a phylogenetic and domain analysis, monocotyledonous CBP family proteins can be subdivided into two groups, namely Group I and Group II. Similarly, dicotyledonous CBP family proteins can be divided into two groups, namely Group A and Group B. High similarities of protein sequences, conserved domains and three-dimensional models were identified among OsHATs and their homologs in Arabidopsis thaliana and maize. Subcellular localization predictions indicated that all OsHATs might localize in both the nucleus and cytosol. Transient expression in Arabidopsis protoplasts confirmed the nuclear and cytosolic localization of OsHAC701, OsHAG702, and OsHAG704. Real-time quantitative polymerase chain reaction analysis demonstrated that the eight OsHATs were expressed in all tissues examined with significant differences in transcript abundance, and their expression was modulated by abscisic acid and salicylic acid as well as abiotic factors such as salt, cold, and heat stresses.
Both monocotyledonous and dicotyledonous CBP family proteins can be divided into two distinct groups, which suggest the possibility of functional diversification. The high similarities of protein sequences, conserved domains and three-dimensional models among OsHATs and their homologs in Arabidopsis and maize suggested that OsHATs have multiple functions. OsHAC701, OsHAG702, and OsHAG704 were localized in both the nucleus and cytosol in transient expression analyses with Arabidopsis protoplasts. OsHATs were expressed constitutively in rice, and their expression was regulated by exogenous hormones and abiotic stresses, which suggested that OsHATs may play important roles in plant defense responses.
KeywordsHistone acetyltransferase Hormone Phylogenetic tree Subcellular localization Rice Stress
Histone modification plays a key role in the regulation of gene expression . Acetylation by histone acetyltransferases (HATs) is normally correlated with increased gene activity, whereas deacetylation via histone deacetylases (HDACs) is often associated with gene repression [2, 3]. In eukaryotes, histone acetylation is catalyzed by five distinct HAT families, which comprise the p300/CREB (cAMP-responsive element-binding protein)-binding protein (CBP) family, the TATA-binding protein-associated factor (TAF)II250 family, the general control non-repressible 5-related N-terminal acetyltransferase (GNAT) family, the MOZ, Ybf2/Sas3, Sas2, and Tip60 (MYST) family, and the nuclear hormone-related HATs family [4, 5]. Bioinformatics analysis suggests that currently there are 12 putative HATs in Arabidopsis thaliana, and these proteins belong to the CBP family (HAC1/PCAT2, HAC2/PCAT1, HAC4/PCAT3, HAC5/PCAT4, and HAC12), the TAFII250-related family (HAF1 and HAF2/TAF1), the GNAT family (HAG1/GCN5, HAG2, and HAG3/ELP3) and the MYST family (HAM1/HAG4 and HAM2/HAG5) [5, 6].
In Arabidopsis, accumulating evidence indicates that HATs contribute to many aspects of plant growth and development, including root development [6, 7], floral development [6, 8, 9], gametophyte development , and cell proliferation during organ growth . In addition, histone acetylation by HATs is important for plant adaptation to environmental changes, such as light signaling [12–15], salt stress , cold stress [17–19], heat stress , abscisic acid (ABA) signaling [16, 21, 22], and other hormone signaling .
Rice is an economically important crop and a model plant for genomics and molecular biology research in monocotyledons. Eight HATs have been identified in rice (OsHATs) and these proteins can be grouped into four major families, namely the CBP (OsHAC701, OsHAC703, and OsHAC704), TAFII250 (OsHAF701), GNAT (OsHAG702, OsHAG703, and OsHAG704), and MYST (OsHAM701) families. The GNAT family can be further divided into three subfamilies, namely GCN5, ELP3 and HAT1 . OsHAG702, OsHAG703, and OsHAG704 belong to the GCN5, ELP3, and HAT1 subfamilies, respectively . Some phylogenetic analyses of HATs have been performed previously [5, 10, 24, 25]. However, the evolutionary relationships of the CBP and TAFII250 families remain unclear. In addition, the biological functions of OsHATs in rice have not been addressed.
To investigate potential functions of OsHATs, systematic bioinformatics and expression analyses were performed. Phylogenetic trees for the CBP and TAFII250 families were generated to explore the evolutionary relationships among representative species of monocotyledons (monocots), dicotyledons (dicots), bryophytes, pteridophytes, animals and fungi. Multiple sequence alignment and domain analysis were used to predict the specific functions of OsHATs in comparison with the HATs of other organisms. We also generated three-dimensional (3D) comparative protein structure models of HATs with the SWISS-MODEL [26–28]. In addition, the subcellular localization of the eight OsHATs was predicted by protein sequence analyses. Transient expression of OsHAC701, OsHAG702, and OsHAG704 in Arabidopsis protoplasts was performed to determine the subcellular localization. Finally, the expression patterns of OsHATs were analyzed using real-time quantitative polymerase chain reaction (PCR) analysis (RT-qPCR). The results obtained will make an important contribution to the elucidation of the functions of different HATs in rice.
Searches of HAT cDNA and protein sequences
List of rice HAT proteins
Protein sequences were aligned with ClustalX 2.1 . Unrooted radial trees were generated with the neighbor-joining method in conjunction with a bootstrap analysis of 1000 replicates using the PHYLogeny Inference Package (PHYLIP) version 3.6 . The Dayhoff PAM model of protein evolutionary changes  was used to measure the branch lengths (evolutionary time) of the tree with the PROTDIST program. TreeView version 1.6.6 was used to display and edit the phylogenetic trees .
Sequence analyses and alignments
Multiple sequence alignments of representative HAT proteins were generated with the ClustalW2 online tool (http://www.ebi.ac.uk/Tools/msa/clustalw2/). Protein domains and function sites of all HAT proteins from rice and other organisms were assigned to the regions of target sequences with InterProScan  using the SWISS-MODEL Workspace website [26–28] (http://swissmodel.expasy.org/workspace/index.php?func=tools_sequencescan1). UniProtKB/TrEMBL (http://www.uniprot.org/blast/) was used to explore further the conserved domains and compositional biases in amino acid sequences . DOG 1.0 was used for drawing protein domain structures . UniProt BLAST (http://www.uniprot.org/blast/) was used to determine the identities of OsHAT proteins and HAT proteins from other organisms . 3D comparative protein structure models of HATs were generated with the automatic modeling mode of SWISS-MODEL [26–28] implemented on the SWISS-MODEL Workspace website (http://swissmodel.expasy.org/). The protein structures were color-coded.
Subcellular localization prediction
SLP-Local (http://sunflower.kuicr.kyoto-u.ac.jp/~smatsuda/slplocal.html) is a subcellular location predictor based on local features of amino acid sequences [36, 37]. TargetP version 1.1 (http://www.cbs.dtu.dk/services/TargetP/) predicts the subcellular localization of eukaryotic proteins from the outputs of ChloroP and SignalP . The protein subcellular localization predictor WoLF PSORT (http://wolfpsort.org/)  is based on PSORTII and iPSORT. Nuclear localization signals (NLS) were discriminated by traditional PSORTII prediction  from the detailed results of WoLF PSORT. The NetNES 1.1 server (http://www.cbs.dtu.dk/services/NetNES/) was used to predict leucine-rich nuclear export signals (NES) in OsHATs by a combination of neural networks and hidden Markov models .
Plant materials and growth conditions
Arabidopsis thaliana cultivar Columbia-0 (Col-0) was used for protoplast isolation and transient expression analyses. Plants were grown in soil in a controlled-environment chamber with a long photoperiod (16 h light/8 h dark) at 22 ± 2°C.
Rice (Oryza sativa L. subsp. japonica cv. Nipponbare) seeds were imbibed with water in the dark at 37 ± 1°C for 24 h and then placed on filter paper (VWR International, Mississauga, ON, Canada) moistened with water in Petri dishes at 23 ± 1°C in the dark. After germination, rice seedlings were grown in beakers containing water, in a culture room with a daily photoperiodic cycle of 9 h light and 15 h dark. The culture room temperature was 23 ± 1°C.
For ABA (Sigma, Oakville, ON, Canada), salicylic acid (SA; Fisher Scientific, Ottawa, ON, Canada), and high salinity treatment, seedlings at the two-leaf stage growing in beakers were transferred to water with or without ABA (100 μM), SA (100 μM) or NaCl (300 mM). For cold treatment, rice seedlings were incubated at 4 ± 1°C in the dark for 3 h. For heat treatment, seedlings were incubated in a 42°C incubator in the dark for 3 h. Seedlings maintained in water at 23 ± 1°C in the dark were used as controls. Leaves of rice seedlings at the two-leaf stage were harvested after treatment, frozen in liquid nitrogen, and stored at −80°C.
Protoplast isolation and transient expression
The full-length cDNAs of OsHAC701 were subcloned into the p2YGW7.0 vector  to create the YFP-OsHAC701 construct, whereas the full-length cDNAs of OsHAG702 and OsHAG704 were subcloned into the pSAT6-EYFP-N1 vector  to generate the OsHAG702-YFP and OsHAG704-YFP constructs. The isolation and transfection of Arabidopsis leaf mesophyll protoplasts were conducted as previously described [44, 45]. Briefly, protoplasts were isolated from well-expanded leaves of 3-week-old Arabidopsis plants. Volumes (10–20 μg) of the OsHAC701/HAG702/704-YFP fusion plasmid and VirD2NLS-mCherry as a nuclear marker  were cotransfected into 150 μl protoplasts (3 × 104 protoplasts) using a PEG–calcium transfection solution. Protoplasts were incubated at 22 ± 2°C under white light overnight to allow expression of the introduced genes. The YFP fluorescence was examined and photographed using a Leica SP5 confocal microscope (Leica, Wetzlar, Germany).
Real-time quantitative PCR analysis
Primer pairs used for RT-qPCR
All RT-qPCR data were expressed as the mean ± standard error. Statistical differences of expression of each OsHAT among rice tissues were assessed by one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) and Student-Neumann-Keuls (SNK) post hoc comparison. The analyses were performed with SPSS 13.0 software (SPSS Inc., Chicago, IL, USA). The threshold of significance was defined as p < 0.05. Student’s t-test was used to assess the significance of differences between the exogenous treatment and the control. Significance was established at p < 0.05 or p < 0.01.
Western blot analysis
Extraction of acid-soluble proteins was performed based on the descriptions by Tariq et al.  and Probst et al. . Fresh rice leaves (0.3 g) were ground in liquid nitrogen and homogenized in 2.25 ml freshly prepared extraction buffer using a Fisher Scientific Model 100 Sonic dismembrator. After centrifugation (15 min, 20,000 rcf, 4°C, twice), the supernatant was stored at −80°C. Protein concentration was determined by the Micro-Bradford Assay with the Bio-Rad Protein Assay Solution. Protein extracts were added to 18.5 mM dithiothreitol, separated on a 16% sodium dodecyl sulfate polyacrylamide electrophoresis gel, and transferred to a polyvinylidene fluoride membrane using a Bio-Rad Semi-dry electrophoretic transfer cell (15 V, 15 min). The following antibodies were used: anti-Histone H3 (1:7500 dilution; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-acetyl-Histone H3K18 (1:10,000 dilution; Cell Signaling), anti-acetyl-Histone H3K9 (1:1000 dilution; Cell Signaling), and anti-acetyl-Histone H4K5 (1:20,000 dilution; EMD Millipore, Billerica, MA, USA). Histone H3 was applied as an equal loading control. The bound immune-complexes were detected with ECL Plus western blot detection reagents (GE healthcare Life Sciences, Baie-d'Urfé, QC, Canada) and exposed to Classic Single-Emulsion Autoradiography Film (Mandel Scientific, Guelph, ON, Canada). The films were developed automatically with an AGFA CP1000 X-Ray Film Processor and scanned with an UMAX Powerlook 1120 scanner.
Results and Discussion
CBP family of HATs
In terms of amino acid compositional bias, OsHAC701 contains a Ser-rich region (residue range 234–247) and a Cys-rich region (1130–1157). OsHAC703 contains two Gln-rich regions (111–135 and 402–625), whereas OsHAC704 contains a Poly-Ser stretch (132–135) as well as a Gln-rich region (379–435). A Cys-rich region in OsHAC701 implies that this protein may interact with other proteins . Gln-rich regions in OsHAC703 and OsHAC704 may mediate transcriptional activation [58, 59]. Similar to OsHAC703, AtHAC1, AtHAC4 and AtHAC12 all contain two Gln-rich regions, whereas AtHAC5 contains one Gln-rich region similar to OsHAC704 and AtHAC2 contains a Ser-rich region similar to OsHAC701.
AtHAC1 shows acetyltransferase activity  and transcriptional coactivator function for a heat-shock-inducible gene in a protoplast system . Furthermore, AtHAC1, AtHAC5 and AtHAC12 have broad-specificity HAT activities and likely act together to acetylate histone H3 lysine 9 (H3K9) . In vivo, AtHAC1, AtHAC5 and AtHAC12 play redundant roles in the promotion of flowering by repressing the expression of the floral repressor FLC (FLOWERING LOCUS C) . Similarly, AtHAC1 was reported to regulate flowering time by epigenetic modification of factors upstream of FLC . Furthermore, AtHAC1 interacts with a tomato heat stress transcription factor HsfB1 in vitro and in vivo . It remains to be determined whether rice and Arabidopsis homologs of CBP family proteins have similar biological functions.
TAFII250 family of HATs
In terms of amino acid compositional bias, OsHAF701 carried an Asp-rich region (18–110) in its N-terminal region, a Poly-Ala stretch (1004–1007) and a Poly-Lys stretch (1635–1645). AtHAF1 contained an Asp-rich region (16–106) near the N-terminus and a Poly-Lys stretch (1289–1296) near the C-terminus similar to OsHAF701. AtHAF2 contained two Poly-Lys stretches (999–1002 and 1195–1201).
TAFII250 family genes encode TATA-binding protein (TBP)-associated factor 1 . The Asp-rich region responsible for substrate binding  overlapped with the TAFII250 TBP-binding domain in OsHAF701 and AtHAF1. The Asp-rich region may therefore participate in the regulation of enzyme activities [64, 65]. A bromodomain known to bind to acetylated histone lysine residues [15, 66] was identified in the C-terminal ends of both TAFII250 and GCN5 family proteins . Sequence alignment showed conservation in the sequences of bromodomains within OsHAF701 and OsHAG702 (see Additional file 3A). In Arabidopsis, both AtHAF2/AtTAF1 and AtHAG1/AtGCN5 are required for H3K9, H3K27, and H4K12 acetylation of light-regulated genes involved in the light regulation of growth and development [12, 15, 62]. In addition, AtHAF2 regulates the expression of several cold-regulated genes independent of its HAT activity . For AtHAF1, RNAi-mediated gene silencing of AtHAF1 in Arabidopsis confers resistance to Agrobacterium-mediated transformation . The similarities in the conserved domains and 3D models of OsHAF701, AtHAF1 and AtHAF2 indicate that OsHAF701 may have similar functions to those of AtHAF1 and AtHAF2.
GNAT family of HATs
GCN5 subfamily of the GNAT family
A GCN5 homolog identified in rice is OsHAG702. Two domains are present in OsHAG702, namely a GCN5-related N-acetyltransferase (GNAT) domain (PS51186, residue range 168–315) and bromodomain (PF00439, 402–491). The 3D models of the GNAT domain and bromodomain analyzed with SWISS-MODEL Workspace [26–28] showed that high similarities existed among OsHAG702, AtHAG1 and ZmHAG101 (Figure 4B). As detected with UniProtKB, OsHAG702 carried three amino acid compositional biases in its N-terminal region (see Additional file 3B): a Ser-rich region (residues 8–67), a Poly-Asp stretch (68–73), and an Ala-rich region (104–124). Similar amino acid compositional biases were identified in other monocot GCN5 subfamily proteins such as ZmHAG101. AtHAG1 also contained a Ser-rich (3–66) region in its N-terminal region.
The Ser-rich region is implicated in the regulation of enzyme activities . The Ala-rich region is essential for transcriptional repression and interaction with TBP (TATA-box binding protein) . Consistent with the analyses of 3D models, alignment of the GCN5 subfamily proteins indicated that the GNAT domain and bromodomain were highly conserved in the GCN5 subfamily proteins of spermatophytes . These results suggest that OsHAG702 might have similar functions to those of AtHAG1 and ZmHAG101. AtHAG1/AtGCN5 shows HAT activity , acetylate primarily H3K14 , and contribute to cold-regulated gene expression [17–19]. AtHAG1 is also involved in controlling floral meristem activity through regulation of the expression of WUSCHEL and AGAMOUS. Furthermore, AtHAG1 is required for light-inducible gene expression [8, 12–15], and is essential for root stem-cell niche maintenance  and the regulation of miRNA accumulation at both transcriptional and posttranscriptional levels . Taken together, these data suggest that AtHAG1 is involved in both long-term epigenetic regulation of chromatin modification and short-term control of transcriptional switches. Given that OsHAG702 displays a high sequence similarity with AtHAG1, OsHAG702 may also play an important role in different aspects of plant development and stress response.
HAT1 subfamily of the GNAT family
Multiple sequence alignments show that the protein sequence of OsHAG704 has high sequence similarity with other HAT1 subfamily proteins in monocots and dicots (see Additional file C3). Domain analysis with InterProScan and 3D protein structure analysis indicated that rice and five other angiosperm HAT1-type proteins all contained a homologous structure called acetyl-coenzyme A: amino acid N-acetyltransferases (Nat) (SSF55729) of about 335 amino acid residues in length. A GNAT domain (PF00583), which was identified in AtHAG2 and GmHAG1204 by domain analysis with InterProScan, was not identified in other HAT1 subfamily proteins. A portion of the GmHAG1206 sequence (61 residues in length) forms a 3D ribbon diagram that is very similar to the GNAT domain of AtHAG2 (Figure 4C). On the basis of the multiple sequence alignment, the GNAT domain sequence in GmHAG1204 showed strong sequence similarity with that of the GmHAG1206 sequence (see Additional file 3C), which suggested that a GNAT domain is present in GmHAG1206. Furthermore, owing to the highly conserved sequences within the HAT1 subfamily proteins of six angiosperms (see Additional file 3C), we predict that OsHAG704 may also contain a GNAT domain.
The high similarities of protein sequences and 3D models among the monocot and dicot homologs of the HAT1 subfamily also suggested that they may have similar functional roles. AtHAG2 (a homolog of OsHAG704) acetylates histone H4K12  and the expression of ZmHAG102/ HAT-B is repressed by ABA treatment during maize seed germination .
ELP3 subfamily of the GNAT family
A high sequence similarity was observed among the ELP3 subfamily proteins in plants , fungi and animals. Analyses of 3D models of ELP3 (TIGR01211) and GNAT (PS51186) domains in OsHAG703, ZmHAG103, ZmHAG107, and AtHAG3 with SWISS-MODEL [26–28] indicated high similarity of 3D structures existed among these proteins (Figure 4D). Sequence alignment (see Additional file 3D) and 3D structure analysis (Figure 4B, C, D) indicated that the GNAT domain in the ELP3 subfamily (OsHAG703) was different from those in the GCN5 subfamily (OsHAG702) and HAT1 subfamily (OsHAG704).
OsHAG703 and its homologs of the ELP3 subfamily may also have similar functions, because they shared high similarities in sequences and 3D structures. AtHAG3/AtELP3 is a member of the conserved Elongator HAT complex that interacts with RNA Pol II during transcript elongation and plays a role in cell proliferation during organ growth . Furthermore, AtHAG3 can regulate plant response to ABA, oxidative stress resistance, and anthocyanin biosynthesis [21, 22]. Arabidopsis Elongator also regulates the auxin signaling gene SHY2/IAA3, the auxin influx carrier LAX2, ethylene signaling, jasmonic acid signaling, and abiotic stress . In addition, RNAi lines of AtHAG3 are resistant to Agrobacterium-mediated transformation .
MYST family of HATs
The MYST family is divided into five unrelated classes. Class I comprises proteins from the green lineage comprising the MYST family proteins of Arabidopsis and rice . OsHAM701 was the only MYST family protein identified in rice. As shown in Figure 4E, the 3D structure of the chromodomain in OsHAM701 was similar to those in AtHAM1, AtHAM2, ZmHAM101, and ZmHAM102. Moreover, the 3D structure of the Nat structure in OsHAM701 was strikingly similar to those in AtHAM1, AtHAM2, ZmHAM101, and ZmHAM102. In addition, OsHAM701 contains an Ala-rich region and a Poly-Gly stretch at its N-terminus (see Additional file 3E).
Amino acid sequences of MYST-type proteins are highly conserved within monocots and dicots . The Nat structure is the main conserved region for acetyltransferase activity. The chromodomain in the MYST family of HATs, similar to the bromodomain in HATs, is reported to be able to identify and bind specific histone residues . The high similarities of amino acid sequences and 3D models of MYST-type HATs suggested that OsHAM701 might have a similar function to those of other MYST-type proteins in Arabidopsis and other angiosperms. AtHAM1 and AtHAM2 preferentially acetylate H4K5  and are involved in gamete formation in both male and female organs in Arabidopsis .
Subcellular localization prediction
Predicted subcellular localization of HATs from rice
SLP-Local a (RI) b
TargetP a (RC) c
WoLF PSORT d
PSORTII (NLS score) e
NetNES: position-residue f
nucl or cyto(1)
531aa, pat4; 419aa, pat7; (0.18)
nucl or cyto(1)
nucl or cyto(4)
756, 1588aa, pat7; (0.22)
82-L, 83-A, 84-K, 85-R, 86-L, 87-E, 88-E, 89-I
nucl or cyto(2)
nucl or cyto(3)
nucl(10.0), pero(2.0), mito(1.0)
71, 744aa, pat4; 741, 1585aa, pat7; (0.55)
nucl or cyto(6)
nucl or cyto(1)
259, 1069, 1266, 1523, 1641-1643aa, pat4; 251, 729, 1523, 1550, 1685, 1686aa, pat7; 1551, 1628aa, bipartite; (4.69)
1782-L, 1783-A, 1784-D, 1785-E, 1786-L, 1787-L, 1788-E, 1789-L
22,23aa, pat4 NLS; (0.03)
nucl or cyto(1)
nucl or cyto(5)
cyto(10.0), chlo(2.0), nucl(1.0)
17-19aa, pat4; 17aa, part7; (0.77)
414-L, 416-R, 417-M, 419-D, 422-L
nucl or cyto(1)
nucl or cyto(4)
nucl(3.5), E.R.(3.0), cysk_nucl(2.5), chlo(2.0), plas(2.0), cyto(1.0), mito(1.0)
14-16aa, pat4; 14aa, pat7; (0.84)
nucl or cyto(5)
nucl(6.0), cyto(4.0), plas(2.0), chlo(1.0)
374aa, pat4 NLS; (−0.29)
ZmHAG101/ZmGCN5 (a homolog of OsHAG702) contains a NLS in its N-terminus. Further studies confirmed that the N-terminus is responsible for nuclear targeting of ZmHAG101 . Earley et al.  reported that AtHAG1 (a homolog of OsHAG702), AtHAG2 (a homolog of OsHAG704), and AtHAM1 and AtHAM2 (two homologs of OsHAM701) tended to be enriched at the periphery of the nucleolus, whereas AtHAM1 and AtHAM2 overlapped with the chromocenters containing the nucleolus organizer region, which suggested that these HATs might be important for the activation of ribosomal RNA genes. On the other hand, ZmHAG102/ZmHAT-B (a homolog of OsHAG704 and AtHAG2) is predominantly localized in the cytosol, and a significant proportion of ZmHAG102 is present in the nucleus . NetNES  detected leucine-rich NES in all OsHATs as well as ZmHAG102. The presence of NES in all OsHATs suggests that they might be exported out of the nucleus.
Nuclear and cytosolic localization of OsHAC701, OsHAG702 and OsHAG704
Expression of OsHATsanalyzed by RT-qPCR
Expression of OsHATs in different tissues
Expression of OsHATs in response to ABA treatment
The plant hormone ABA plays a pivotal role in a variety of developmental processes such as regulation of seed germination and seedling establishment . ABA is also involved in plant responses to various stresses, such as salt, osmotic, cold, wound and pathogenic stresses [16, 80, 81]. To determine if the expression of Os HATs during rice seedling growth was regulated by ABA, we analyzed the expression pattern of OsHATs after treatment with 100 μM ABA for 24 h (Figure 6B). PR10a was used as a positive control. PR10a transcription increased by 2.55-fold after ABA treatment for 24 h, which indicated that the treatment was effective. Transcript levels of OsHAC701, OsHAC703, OsHAG702, OsHAG703, and OsHAM701 were significantly elevated in response to exogenous ABA application. These results indicated that OsHAC701, OsHAC703, OsHAG702, OsHAG703, and OsHAM701 may be involved in the ABA signaling pathway for response to environmental stresses during rice seedling growth.
Treatment with ABA for a short period of time (0–120 min) causes dynamic changes in histone H3 and H4 acetylation and phosphorylation in tobacco BY-2 cells, Arabidopsis T87 cells and whole leaves of tobacco and Arabidopsis . Previous studies reveal that treatment of the leaves of rice seedlings with 100 μM ABA for 24 h increases the expression of OsHDA702, but represses the expression of OsSRT701 and OsSRT702 . ABA treatment of barley seedlings also induces the expression of HvGCN5 HvELP3, and HvMSYT . In contrast, ABA treatment of maize seeds for 48 or 72 h represses the expression of ZmHAG101 (ZmGCN5) and ZmHAG102 (ZmHAT-B), as well as several ZmHDACs. ABA selectively induces histone acetylation of the VP1 gene (the embryogenesis-related gene viviparous1) and activates its transcription, which suggests that ZmHATs and ZmHDACs might participate in the ABA signal pathway during seed germination . In Arabidopsis seedlings, ABA downregulates the expression of AtHD2C, an HD2-type HDAC, whereas overexpression of AtHD2C enhances the ABA tolerance of Arabidopsis, which suggests that AtHD2C may modulate ABA and stress responses . Furthermore, the involvement of AtHAG3 (ELP3) in ABA responses in Arabidopsis is reported [21, 22]. More recently, Chen et al.  reported that ABA treatment enriched the gene activation markers, histone H3K9K14 acetylation, and H3K4 trimethylation, but decreased the gene repression marker, H3K9 dimethylation, of a number of ABA-inducible genes in Arabidopsis. Taken together, these observations indicate that histone acetylation/deacetylation induced by HATs and HDACs is involved in the regulation of the ABA signaling pathway in a variety of plants including Arabidopsis, tobacco, maize, and rice.
Expression of OsHATs in response to SA treatment
SA plays an important regulatory role in multiple physiological processes such as the plant immune response. Furthermore, SA can interact with other phytohormones such as ABA, auxin, and gibberellic acid [83, 84]. RT-qPCR analysis was performed using total RNA from leaves of two-leaf-stage seedlings exposed to 100 μM SA to examine whether OsHATs were regulated by SA (Figure 6C). The transcript level of PR10a increased by 1.58-fold in response to SA treatment for 24 h compared with that of the control plants. After SA treatment for 36 h, the expression levels of OsHAC703 and OsHAC704 were reduced to 67.43% and 42.76%, respectively, of those of control plants. No obvious changes were observed in the expression of the other six OsHAT genes after treatment with SA for 24 h or 36 h (data not shown). Previous studies show that treatment of the leaves of rice seedlings with 100 μM SA for 12 h downregulates the expression of OsHDA702, OsHDA704, OsHDA706, OsSRT701, and OsSRT702 but upregulates the expression of OsHDT702 . These observations indicate that members of the HAT and HDAC gene families may be involved in the SA signaling pathway in plant defense responses.
Expression of OsHATs in response to salt, cold and heat treatment
With salt treatment, the transcript levels of OsHAC701, OsHAC703, OsHAC704, OsHAG703, and OsHAM701 were increased by 36.02%, 23.41%, 94.11%, 49.57%, and 170.32%, respectively, in comparison with the controls (Figure 6D).
The expression of eight OsHATs was also measured in seedlings exposed to low temperature. Except for OsHAG704, the expression of the other OsHATs was decreased to 45.84%, 42.20%, 13.32%, 45.17%, 34.81%, 53.05% and 34.16%, respectively, after 3 h of cold exposure, in comparison with the controls (Figure 6E).
In response to heat treatment (Figure 6F), PR10a transcripts increased by 2.39-fold in leaves of two-leaf-stage seedlings after exposure to 42°C for 3 h. Compared with the controls, the transcript levels of OsHAC701, OsHAG702, and OsHAM701 were increased by 58.97%, 75.77% and 31.84%, respectively, in response to heat treatment. Conversely, the expression of OsHAC704 and OsHAG704 were decreased by 39.99% and 71.92%, respectively.
Previous research shows that salt stress induces the expression of OsHDA702, whereas the expression of OsHDA704, OsHDA712, and OsSRT702 is decreased. Cold treatment represses the expression of OsHDA704, OsHDA712, and OsSRT701 but induces the expression of OsHDA702. AtHAG1 contributes to the expression of cold-regulated genes during cold acclimation [17–19]. AtHAF2 participates in the regulation of some cold-regulated genes . AtHAC1 interacts with a tomato heat stress transcription factor HsfB1 that contains a histone-like motif . In addition, an analysis of microarray data with Genevestigator indicated that heat stress (38°C for 3 h) upregulates the expression of AtHDA6, AtHDA7, AtHDA5, AtHDA8, and AtHDA14. Taken together, HATs and HDACs can be modulated by salt, cold or heat stress, which suggests that these proteins may play an important role as epigenetic regulators for plant response to abiotic stress conditions.
With regard to the leaves of two-leaf-stage rice seedlings treated with salt for 12 h, western blot analysis showed that histone H3K18 acetylation was increased (see Additional file 5B), but no changes in the acetylation of H3K9 and H4K5 (Additional file 5C and D) were observed. Previous studies show that in cultured cells and leaves of Arabidopsis and tobacco, histone H3 Ser-10 phosphorylation, H3 phosphoacetylation and histone H4 acetylation are upregulated by salt treatment . We observed increased transcript levels of OsHAC701, OsHAC703, OsHAC704, OsHAG703, and OsHAM701 in response to salt stress, which was correlated with the increase in histone H3K18 acetylation. Therefore, these proteins may have a role in salt stress response in rice.
The present phylogenetic analyses of the CBP and TAFII250 HAT family provide insights into the evolutionary relationships of these two protein families. Both monocot and dicot CBP family proteins can be subdivided into two distinct groups. Diversity in the specific domains identified in different OsHATs indicates that OsHATs have undergone functional diversification. The high similarities of protein sequences, conserved domains and 3D models among OsHATs and their homologs in Arabidopsis and maize suggests that OsHATs perform multiple functions during rice growth and development. Subcellular localization predictions indicate that all OsHATs might localize in both the nucleus and cytosol. Transient expression analyses of Arabidopsis protoplasts confirmed the nuclear and cytosolic localization of OsHAC701, OsHAG702, and OsHAG704. RT-qPCR analyses show that OsHATs are expressed constitutively in rice. In addition, their expression is modulated by exogenous treatment with the hormones ABA and SA as well as salt, cold and heat stresses, which suggests that OsHATs may play important roles in plant defense responses.
We thank Frédéric Marsolais for providing RT-qPCR and western blot equipment. We thank Abdelali Hannoufa for offering acetyl-Histone H4K5 antibody. We thank Susan Sibbald for English editing of the manuscript. We acknowledge the technical help and support of Sukhminder Sawhney, Xinhua Wang, Farida Meerja, Fuqiang Yin, Agnieszka Pajak, Ying Wang, Mimmie Lu, Fang Hui and Alex Molnar at the Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada. We appreciate the help of Guoliang Li, Lianyu Yuan, Linmao Zhao and Yan Gao at the South China Botanical Garden, Chinese Academy of Sciences. This work was supported by grants from the Guangdong Science and Technology Projects (2011A020102008), the National Natural Science Foundation of China (nos. 30871465, 30971564 and 90919038), the National High Technology Research and Development Program of China (no. 2008AA10Z107), Agriculture and Agri-Food Canada research funding, and the National Science Council of Taiwan (99-2321-B-002-027-MY3 and 101-2923-B-002-005-MY3). XL was supported by the China Scholarship Council.
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