Figure 8

Expression of antioxidant enzymes in transgenic lines. (A) The expression of antioxidant enzymes under normal conditions. (B) The expression of antioxidant enzymes during MV treatment. Also, qPCR analysis was performed to detect the levels of the antioxidant enzymes (NtSOD, NtGPX, NtAPX1, NtAPX2, NtCAT1 and NtCA). Approximately three-week-old transgenic and wild-type tobacco plants were used for the expression analysis. For the MV treatment, the tobacco seedlings were sprayed with 0.5 mM MV and analyzed 6 h after treatment. The data are presented as the mean ± standard error of three independent experiments. The values indicated by the different letters are significantly different at P < 0.05, as determined using Duncan’s multiple range tests.