Figure 7
Effect of PAO inhibitor, guazatine acetate (Guazatine), on canker disease susceptibility of the transgenic line (TG9). (A-C) PAO enzyme activity (nmol acetylspermine/min/mg protein, A), DAB staining (B) and hypersensitive response (C, shown by arrows) of leaves treated with Guazatine or water (H2O), collected on 3 DPI. (D) Representative photographs showing symptoms of Guazatine or H2O-treated leaves after Xac inoculation for 9 d. (E-F) Disease index (E) and lesion size (data of 9 DPI, F) of the leaves treated with Guazatine or H2O after Xac infection. * and ** indicate the values are significantly different compared with WT at significance level of P < 0.05 and P < 0.01, respectively.