Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL

BMC Plant Biology

Table 4 Estimation of relative turnover potential for enzymes of artemisinin biosynthesis using the 2^-ΔΔCTmethod [42]

Enzyme	Substrate	K_m (μM)	k_cat (s^-1)	ΔΔC_T	Normalized transcript amount relative to *RED1*2^-ΔΔCT	relative turnover	Reference for kinetic constants
FDS1	IDP	29.4	0.7	-7.1	137	96	[32]
	GDP	17.2
ADS	FDP	2	0.004	-8.4	338	1.4	[54]
DBR2	AA	19	2.6	-7.9	239	621	[15]
ALDH1	DHAA	8.8	7.7	-5.9	60	462	[16]
	AA	2.6	1.5	-5.9	60	90
RED1	DHAA	67	0.28	0.0	1	0.3	[18]

β-Actin was used as reference gene. The C_T-values for flower buds (Table 2) were used for the calculations. The RED1 gene was used for the normalization of values. Substrates: AA, artemisinic aldehyde; AAOH, artemisinic alcohol; AD, amorpha-4,11-diene; DHAA, dihydroartemisinic aldehyde; FDP, farnesyldiphosphate; GDP, geranyldiphosphate; IDP, isopentenyldiphosphate.

ISSN: 1471-2229