Figure 3

Localization of anticlinal microtubules within the expanding pavement cell lobes. (A) Microtubules in a single fixed cell detected using freeze shattering and immunolocalization. Regions of interest in are labeled a' xy view and b' xy view. Insets are projections of the xz and yz views of subregions a' and b', respectively. *, indicates the location of the adaxial periclinal surface of the cell in the xz and yz views. (B) Microtubules in a living pavement cell detected using microprojectile bombardment of the GFP:TUB6 expression construct. Regions of interest are labeled c' xy view and d' xy view. Insets are projections of the yz and xz views of subregions c' and d', respectively. *, indicates the location of the adaxial periclinal surface of the cell in the yz and xz views. Bar = 10 μm