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Figure 3 | BMC Plant Biology

Figure 3

From: The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress

Figure 3

Activity of the grape VvMYB60 promoter is localized to guard cells in Arabidopsis, and is down-regulated by ABA. (A) 15-day-old pVvMYB30:GUS seedling. (B) Magnification of leaf primordia in (A), showing staining at the base of trichomes. (C) Detail of an emerging later root. (D) 15-day-old pVvMYB60:GUS seedling. (E) Magnification of leaf primordia in (D), showing staining of differentiating stomata. (F) Detail of a pVvMYB60:GUS mature leaf, showing staining of fully differentiated stomata. (G) qPCR analysis of GUS expression in response to 100 μM ABA, in two independent pVvMYB60:GUS lines (mean ± SD, n = 3). A transgenic line carrying the 1.3 kb Arabidopsis MYB60 promoter fused to GUS (pAtMYB60:GUS) was used as a control. Total RNA samples were extracted at the time points indicated (hours). Relative GUS transcript levels were determined using gene-specific primers and normalized to the expression of the AtACTIN2 gene (At3g18780). Asterisks indicate values significantly different from the untreated control (P < 0.001, t-test). (H) and (I) Histochemical analysis of GUS expression in pVvMYB60:GUS leaves in response to ABA. (H) GUS staining of stomata in a control leaf. (I), GUS staining of stomata following 6 hours of exposure to 100 μM ABA.

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BMC Plant Biology

ISSN: 1471-2229

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