Figure 5

The transient expression of NRP-A and NRP-B induces GmNAC6 expression. A. The expression of GmNAC6 in soybean protoplasts from suspension cells. Soybean protoplasts were electroporated with the 35S::YFP-NAC6 construct or the empty vector, and the expression of GmNAC6 and YFP-GmNAC6 was monitored by qRT-PCR. The values represent the mean ± SD of three replicates from two independent experiments. B. The transient expression of YFP-GmNAC6 does not impact NRP-A or NRP-B transcript accumulation. Soybean protoplasts were electroporated with the 35S::YFP-NAC6 construct (dark gray) or the empty vector (light gray), and the expression of NRP-A and NRP-B was determined by qRT-PCR. The values represent the mean ± SD of three replicates from three independent experiments. Asterisks indicate values significantly different from the control treatment (p < 0.05, Tukey HSD test). C and D. The expression of NRP-A and NRP-B in soybean protoplasts. Plasmids containing NRP-A (C) or NRP-B (D) expression cassettes were electroporated into soybean protoplasts, and the transient gene expression was monitored by quantitative RT-PCR as in (A). E. The specific induction of GmNAC6 by NRP-A or NRP-B transient expression. Plasmids containing NRP-A (light gray) or NRP-B (dark gray) expression cassettes were electroporated into soybean protoplasts, and the relative expression of NAC genes was monitored by qRT-PCR. The relative quantitation of expression was calculated using 2^-ΔΔCt method. The values are relative to the control treatment (empty vector), and asterisks indicate those significantly different from the control treatment (p < 0.05, Tukey HSD test).