Induction of stromule formation by extracellular sucrose and glucose in epidermal leaf tissue of Arabidopsis thaliana
© Schattat and Klösgen; licensee BioMed Central Ltd. 2011
Received: 25 April 2011
Accepted: 16 August 2011
Published: 16 August 2011
Stromules are dynamic tubular structures emerging from the surface of plastids that are filled with stroma. Despite considerable progress in understanding the importance of certain cytoskeleton elements and motor proteins for stromule maintenance, their function within the plant cell is still unknown. It has been suggested that stromules facilitate the exchange of metabolites and/or signals between plastids and other cell compartments by increasing the cytosolically exposed plastid surface area but experimental evidence for the involvement of stromules in metabolic processes is not available. The frequent occurrence of stromules in both sink tissues and heterotrophic cell cultures suggests that the presence of carbohydrates in the extracellular space is a possible trigger of stromule formation. We have examined this hypothesis with induction experiments using the upper epidermis from rosette leaves of Arabidopsis thaliana as a model system.
We found that the stromule frequency rises significantly if either sucrose or glucose is applied to the apoplast by vacuum infiltration. In contrast, neither fructose nor sorbitol or mannitol are capable of inducing stromule formation which rules out the hypothesis that stromule induction is merely the result of changes in the osmotic conditions. Stromule formation depends on translational activity in the cytosol, whereas protein synthesis within the plastids is not required. Lastly, stromule induction is not restricted to the plastids of the upper epidermis but is similarly observed also with chloroplasts of the palisade parenchyma.
The establishment of an experimental system allowing the reproducible induction of stromules by vacuum infiltration of leaf tissue provides a suitable tool for the systematic analysis of conditions and requirements leading to the formation of these dynamic organelle structures. The applicability of the approach is demonstrated here by analyzing the influence of apoplastic sugar solutions on stromule formation. We found that only a subset of sugars generated in the primary metabolism of plants induce stromule formation, which is furthermore dependent on cytosolic translational activity. This suggests regulation of stromule formation by sugar sensing mechanisms and a possible role of stromules in carbohydrate metabolism and metabolite exchange.
Stromules (stroma filled tubules)  are protrusions of the plastid envelope with a diameter of usually less than 1 μm . These filament-like structures are highly dynamic and can extend and retract within seconds . Although tubules extending from the plastid surface had been described in a monograph about plastids in 1908 (see ), their significance and morphological relevance was recognized only after development and improvement of suitable fluorescence microscopy techniques. In particular, the generation of transgenic plants expressing chimeric proteins consisting of green fluorescent protein (GFP) fused to chloroplast targeting transit peptides allowed the first detailed analysis demonstrating the presence of stromal proteins within these structures . Over the past years, stromules were found in a variety of vascular plant species, non-vascular plant species and green algae (as summarized in ) which suggests evolutionary conservation of these structures and implies that they might play an important role in all members of the Viridiplantae. Despite significant progress in understanding the importance of certain cytoskeleton elements and motor proteins for stromule maintenance [6–8], the function of stromules remains elusive. One way to approximate their role in plant cells is to search for physiological conditions which lead to the induction of stromules.
Stromules are found at relatively high frequency, for example, in sink tissues like ripening tomatoes , in leaf samples placed on sucrose-rich medium as well as in BY2 cell cultures . In all these instances, the cells showing high stromule frequency are exposed to a relatively high concentration of carbohydrates which suggests a link between the presence of sugars and stromule formation. Here, we have tried to elucidate this potential correlation by measuring the influence of exposure to different sugar solutions on stromule frequency in a model plant tissue, notably the upper leaf epidermis of Arabidopsis thaliana.
We found in our experiments that stromule formation is strongly induced in epidermal plastids after application of sucrose and glucose. The specificity of this induction is confirmed by the inability of either fructose, sorbitol and mannitol to induce the same reactions. Furthermore, stromule induction by sucrose and glucose is not restricted to epidermal plastids but can likewise be observed in chloroplasts of the palisade parenchyma.
Upper leaf epidermis as suitable model tissue for stromule induction
Stromule frequency is not influenced by cell size
To identify changes in the chosen model tissue a suitable stromule parameter is needed. Stromule frequency (SF), i.e. the proportion of plastids showing at least one stromule, has been previously used for quantification of changes induced by experimental treatments to epidermal cells of Nicotiana benthamiana  and occurring during ripening of tomato fruit . Because SF is a proportion, Waters and colleagues  introduced the 'stromule index' for statistical analysis and graphical display, which represents the arcsin transformed SF (arcsin √ x, where as × is the proportion). SF, as it has been used before, is based on the comparison of SF estimated for individual cells. This necessitates the cell context to be considered and requires imaging of plastids and cell boundaries. Not having to consider the cell context would allow for streamlined imaging and data analysis but would presuppose that cells of different size do not differ in SF. Our measurement of the size of 1023 cells from 3 leaves (as measure of cell size the surface area bound by the lateral cell wall was used) shows that the upper epidermis of Arabidopsis thaliana is composed of cells of very different size (ranging in our measurements from 15 μm2 to 7044 μm2) with small cells dominating the epidermis by number (Figure 1B and additional file 1 panel A). As illustrated in Figure 1C, the number of plastids per cell (ranging in our measurements from 1 to 20) is positively correlated with cell size (coefficient of determination r2 0.8016 and p < 0.0001). In order to test if stromule frequency changes with cell size, cells were pooled into size classes incrementing by 497 μm2. The respective SF was calculated by dividing the total number of plastids exhibiting stromules by the number of all plastids within a given size class. As Figure 1D shows, a correlation of cell size and SF is unlikely in this tissue (r2 0.009; p 0.1939; because of unequal size of size class ' > 4437' this class has not been considered for regression analysis). Taking this into account, stromule frequency in the upper epidermis can be estimated without considering the cell context, which makes imaging cell boundaries unnecessary.
Stromule frequency varies in untreated leaves
In order to determine the variability of SF in untreated tissue, SF of 109 leaves from different plants was estimated. The box plot shown in Figure 1E illustrates that 50% of the samples had a SF ranging from 0.13 to 0.23 with a median of 0.18, while in few cases (< 20 plants) leaves showed markedly deviating frequencies (< 0.09 or > 0.27). By using different leaves for different time points in a time course experiment, such variation could mask potential inducing effects. Therefore, we used leaf squares from a single leaf for a given time course experiment.
Stromule formation is induced by extracellular sucrose
The most prominent transport sugar present in the phloem sap of plants is sucrose  which is usually unloaded from the phloem into the apoplast of the sink tissue. Sucrose is also the main carbon source in media used for plant tissue culture. In both types of cells, stromules have been observed in high abundance [9, 10] which suggests that the presence of sucrose in the apoplast of plant cells might support stromule formation. In order to experimentally test this hypothesis, we infiltrated leaves of Arabidopsis thaliana with a buffer solution (APW) supplemented with 40 mM sucrose, a concentration routinely used in Arabidopsis thaliana tissue culture medium.
Like other sucrose-induced physiological reactions, e.g. anthocyanin accumulation in Arabidopsis thaliana seedlings or alpha-amylase induction in barley embryos [15, 16], the increase in SF is concentration dependent. Infiltrating tissue samples with solutions of 1 mM, 10 mM or 40 mM sucrose suggests correlation of SF and sucrose concentration of the infiltration medium (Figure 2D; for absolute values of stromule index and stromule frequency see additional file 1 panel E and F). However, a further increase in sucrose concentration (> 40 mM) did not lead to an additional increase in SF suggesting a kind of saturation effect. Furthermore, we have never observed SF exceeding 60%, i.e. even under "optimal" inducing conditions approximately 40% of all plastids in a sample are yet devoid of visible stromules.
Induction of stromule formation is not an osmotic effect
In order to elucidate whether sucrose induction of stromule formation is merely an osmotic effect, the experiments were repeated with solutions of mannitol and sorbitol, which are both not part of the primary carbon metabolism and are routinely used to apply osmotic stress [17–20]. In neither case did vacuum infiltration result in significant increase in SF (Figure 2A). Instead, infiltration of sorbitol led even to a mild inhibition of stromule formation demonstrating that changes in osmotic conditions cannot be the reason for the stromule induction observed in the presence of sucrose. This suggests that stromule formation can be elicited specifically by sugars present during primary carbon metabolism.
However, not even all of these sugars are capable of inducing stromule formation. If sucrose is replaced by either glucose or fructose in the infiltration experiments only glucose was able to induce stromule formation, whereas fructose treatment did not lead to any change in SF (Figure 2A). Although the process is not well understood, it is widely accepted (based on supporting experimental evidence, summarized in ) that extracellular fructose generated by sucrose-cleavage in the apoplast is imported into the cell. Considering that intracellular fructose can be converted to phosphorylated glucose, stromule induction is probably not caused by an overall increase in cell metabolic activity but likely depends on specific metabolic and/or signaling pathways.
Stromule formation requires de novoprotein synthesis in the cytosol
The apparent influence of metabolic activity on stromule formation was further analyzed by sucrose induction experiments performed in the presence of inhibitors of protein biosynthesis. If the sucrose solution is additionally supplemented with cycloheximide (CHX), which inhibits the activity of 80S ribosomes and thus is used to prevent translation in the cytosol [22, 23], we observed complete inhibition of stromule formation (Figure 2E). In contrast, control experiments performed with sucrose solutions supplemented with 0.03% DMSO, the solvent of CHX, did not show any inhibitory or inducing effect (Figure 2E) confirming the specificity of this reaction. On the other hand, neither streptomycin nor spectinomycin, which are used to prevent translation within plastids by inhibition of the 70S ribosomes [22, 24–26], affected sucrose-triggered stromule induction (Figure 2E). This indicates that de novo synthesis of nuclear encoded proteins but not of those encoded by the plastid genome is required to mediate the signal from apoplastic sucrose accumulation to stromule formation.
Sucrose-dependent stromule formation is observed also in the palisade parenchyma
In the present study, we aimed to establish an experimental system facilitating the reproducible induction of stromule formation in living plant tissue in order to make these enigmatic structures better accessible to systematic investigation. A possible connection between extracellular sugars and stromule formation has been suggested by several reports concerning high stromule frequency in heterotrophic cell cultures and sink tissues. Using the upper leaf epidermis of a transgenic Arabidopsis thaliana line harboring green fluorescent plastid stroma as model tissue, we addressed the influence of extracellular sugars on stromule formation.
Stromule formation is specifically induced by sucrose and glucose
We found that formation of stromules is specifically induced only by a subset of sugars generated in plants. While vacuum infiltration of either sucrose or glucose leads to a significant increase in SF, an inducing effect of fructose or mannitol cannot be observed. Infiltration of sorbitol leads even to a mild inhibition of stromule formation. Thus, our data suggest that stromule formation is most likely due to neither osmotic effects nor the result of the presence of metabolizable sugars in general. Instead, it seems that specific signaling pathways involving sucrose and/or glucose play a role in the induction process.
The role of sucrose in signal transduction is difficult to evaluate despite the fact that there is strong evidence for sucrose-specific intracellular and extracellular sensing mechanisms operating in plants [15, 27]. Since sucrose is efficiently cleaved into fructose and glucose, both in the apoplast and in the cytosol by invertases and sucrose synthase, the signaling function of sucrose is difficult to distinguish from that of its cleavage products - glucose or UDP-glucose. Glucose sensing, on the other hand, is already understood in some detail. In particular, the intracellular enzyme hexokinase1 (HXK1) has been identified as a key player in this process. Beside its enzymatic activity, HXK1 is an important glucose sensor. Isoforms of this enzyme are present within plastids as well as associated with mitochondria. The latter isoform is also found in the nucleus where it is part of a protein complex involved in gene regulation . In addition to HXK1, further potential glucose sensors have been reported, which alternatively or additionally might be involved in glucose induced stromule formation .
At this stage, it is not known if sucrose and glucose can act as independent signals for stromule formation or if the sucrose induction observed is caused by the release of glucose after sucrose cleavage. Likewise, the question remains to be answered as to whether the stromule inducing signal is sensed extracellularly or intracellularly.
It should be kept in mind that changes in extracellular sugar levels might not only influence the carbohydrate metabolism of a cell but may be a cause of stress for the plant cells potentially leading to the induction of stress signaling pathways . Further experimental evidence is therefore required to substantiate the presumed interdependence of stromule formation and carbohydrate metabolism. Hence, our next experiments will address the question if glucose and sucrose generate independent stromule inducing signals and if internal changes in sugar levels are sufficient to change stromule frequency (making use, for example, of non-metabolizable glucose and sucrose analogues as well as mutants with altered intracellular sucrose and glucose levels).
Stromules may support metabolite exchange
Although several possible functions for stromules have been suggested and discussed , the final role of stromules in plant cells remains still enigmatic. The observation of stromules or other envelope protrusions being in direct contact with mitochondria and peroxisomes led to the suggestion that formation of envelope protrusions, like stromules, supports photorespiration [30–33] by increasing the interactive surface between the organelles and, in turn, facilitating efficient metabolite exchange. However, experimental evidence that these organellar connections become more frequent under photorespiratory conditions, which would support this assumption, is yet missing.
Alternatively, the increase in interactive plastid surface by stromule formation may have more general consequences on the interaction of plastids with the cytosol or other organelles, which might be particularly relevant under conditions of increased demand of metabolite import or export across the plastid envelope membranes [2, 5]. Indeed, our results demonstrating stromule induction by sucrose and glucose seem to support this hypothesis. Apoplastic glucose and/or sucrose are particularly prominent in sink tissue and heterotrophic cell cultures. The non- or less-photosynthetically active plastids of these cells import large amounts of glucose-6 phosphate from the cytosol in order to generate the ATP and NADPH needed to fulfill their metabolic functions, which in turn originates from extracellular sucrose or glucose pools. On the other hand, triose phosphates, which are simultaneously produced in this process, are exported back into the cytosol. This continuous need for import and export of metabolites in heterotrophic tissue might explain the high stromule abundance in BY2 cells as well as in non-green tissue like ripening tomato fruits and dark grown seedlings. Furthermore, it could explain why chloroplasts, which generate ATP and NADPH by photosynthetic processes, are reported to show generally lower stromule frequencies than non-green plastids . The fact that chloroplasts develop stromules to a similar extent as epidermal plastids after vacuum infiltration of glucose or sucrose seems to be contradictory at first glance. However, it is well established that under high sugar conditions source activity is suppressed and sink activities are triggered . Naturally, this change occurs during fruit development , a process that in tomato fruits goes along with an increase in stromule frequency and length . Furthermore exposure to extracellular glucose and sucrose induces major changes in gene expression [36, 37]. Such a change might thus take place also by our sucrose and glucose treatments, since the cycloheximide experiments demonstrate the requirement of de novo protein synthesis for stromule induction.
While up to now only speculations about stromule related processes were possible, the present study provides experimental evidence, which suggests a possible involvement of stromules in carbohydrate metabolism. This supports the idea of stromules being involved in optimizing metabolite exchange. The stromule inducing capacity of glucose and sucrose, important metabolites and signal molecules, provides experimental evidence for the involvement of a typical sugar sensing mechanism in stromule regulation. However, the sugar sensing mechanism and signaling cascades involved remain still unknown and require further investigation. Our model system, the upper leaf epidermis of Arabidopsis thaliana, may provide a useful tool for solving these questions.
Chemicals and solutions
All chemicals were purchased from Sigma-Aldrich (Deisenhofen, Germany), Roth (Karlsruhe, Germany), or Serva (Heidelberg, Germany). As buffer for dissolving and diluting sugars and inhibitors, artificial pond water (APW)  was used. All solutions were prepared immediately before use.
Microscopy, hardware and software
For imaging of EGFP fluorescence, an Axioscop 2 upright microscope (Carl Zeiss, Jena, Germany) operating in epifluorescence mode (fluorescence filter 'endowGFP' F41-017 purchased from AHF Analysetechnik, Tübingen, Germany) was used. Images were captured using either an Axiocam HRc camera (Carl Zeiss, Jena, Germany) or a KY-F75 camera (JVC, Japan). Microscope, camera and piezo stepper were controlled by either of the frame grapping software packages AxioVision (Carl Zeiss, Jena, Germany) or DISKUS (Hilgers, Königswinter, Germany).
Plant material, sample preparation and drug treatments
Transgenic Arabidopsis thaliana plants constitutively expressing the chimeric protein FNR/EGFP, which consists of the chloroplast targeting transit peptide of ferredoxin-NADPH-oxidoreductase (FNR) fused to an enhanced derivative of the green fluorescent protein (EGFP), were grown on soil at 120 μEinstein m-2s-1 and 60% relative air humidity under a short-day light regime (8 h light/16 h dark). For vacuum infiltration, expanding leaves from 10 - 12 week old plants, which had reached approximately 75% of the size of mature leaves, were harvested. After removing the mid vein, the leaves were cut into four squares and vacuum infiltrated using a 5 ml or 10 ml syringe and a 2 ml reaction tube. The tube was filled with 1.5 ml of the respective solution and a 10 ml syringe was placed on top of the tube. By pulling the plunger of the syringe, vacuum was applied for not longer than 2s. Upon release, the resulting negative pressure in the tissue caused the liquid to flood the intercellular space. The infiltrated leaves were immediately analyzed or further incubated. For treatment of leaf samples with inhibitors of translation, samples were infiltrated with APW supplemented with either 100 μM cycloheximide, 100 μg ml-1 spectinomycin, or 100 μg ml-1 streptomycin and incubated for one hour in darkness. Then the solutions were replaced by APW supplemented with 40 mM sucrose in addition to the respective inhibitor. As a solvent control for cycloheximide treatment, APW was supplemented with DMSO at 0.03%. Each experiment was performed at least three times with leaves of different plants.
Imaging and data processing
After vacuum infiltration leaf squares were either immediately analyzed (time point 0 h) or incubated at room temperature in the dark for the given time periods (1, 2, or 3 h). For each time point, epidermal plastids of 6 individual leaf sectors were imaged by capturing an image series along the z-axes. The resulting image stack was further processed using the software package AxioVision (Carl Zeiss, Jena, Germany). Image stacks were processed into one 'stacked' 2D image with the help of CombineZP  as described previously . After import of the stacked images into the ImageBrowser package (Carl Zeiss, Jena, Germany), plastids with and without stromules were marked following a color code. The resulting image layer, which consisted solely of markings, was exported as an image file. Markings in these images were automatically counted using the Photoshop plug-in FoveaPro 4 (Reindeer Graphics, Asheville, USA). Data files produced with FoveaPro 4 were analyzed with Excel (Microsoft, Redmond, Washington, USA).
Calculating stromule frequency
The values for SF were calculated as followed. For a time point of a time course experiment image stacks at 6 different spots per leaf square were taken as described (capturing approx. 250 epidermal plastids per spot, i.e. approx. 1500 plastids per leaf square). For each leaf square stromule frequencies of the six spots were calculated resulting in six SF values for each leaf square (for estimating the SF in the palisade parenchyma, only chloroplasts which were completely visible in the taken image stacks were considered). Afterwards SF values were arcsin transformed (arcsin √ SF) according to Waters et al.  resulting in stromule index (SI) values. To summarize the data of experimental repeats, for each experiment the arithmetic average and the 99% confidence intervals were calculated using SI values (additional file 1 panel C, E and additional file 3 panel A).
These average SI values and confidence intervals have been converted back into SF values by calculating the square of the sinus of the SI values ((sin SI)2) for ease of conveyance. Bar charts of stromule index as well as back-transformed data are shown in additional file 1 panel C-F and additional file 3 panel A and B. For better comparison of the effect of different treatments, the increase or decrease in relation to the initial stromule frequency was plotted in the graphs presented in Figure 2A, D, E and 3A.
We thank Jaideep Mathur and Sebastian Schornack for helpful discussions and comments on the manuscript; Naomi Marty and Michael Wozny for helping with English wording; Martin Paulmann, Max Paulmann and Armin Danziger for their kind support in marking plastids. This work was supported by grants from the state Sachsen-Anhalt (Exzellenznetzwerk Biowissenschaften).
- Köhler RH, Cao J, Zipfel WR, Webb WW, Hanson MR: Exchange of protein molecules through connections between higher plant plastids. Science. 1997, 276 (5321): 2039-2042. 10.1126/science.276.5321.2039.PubMedView ArticleGoogle Scholar
- Hanson MR, Sattarzadeh A: Stromules: Recent Insights into a Long Neglected Feature of Plastid Morphology and Function. Plant Physiology. 2011, 155 (4): 1486-1492. 10.1104/pp.110.170852.PubMedPubMed CentralView ArticleGoogle Scholar
- Gunning BE: Plastid stromules: video microscopy of their outgrowth, retraction, tensioning, anchoring, branching, bridging, and tip-shedding. Protoplasma. 2005, 225 (1-2): 33-42. 10.1007/s00709-004-0073-3.PubMedView ArticleGoogle Scholar
- Gray JC, Sullivan JA, Hibberd JM, Hansen MR: Stromules: Mobile Protrusions and Interconnections Between Plastids. Plant Biol. 2001, 3 (3): 223-233. 10.1055/s-2001-15204.View ArticleGoogle Scholar
- Hanson MR, Sattarzadeh A: Dynamic morphology of plastids and stromules in angiosperm plants. Plant Cell Environ. 2008, 31 (5): 646-657. 10.1111/j.1365-3040.2007.01768.x.PubMedView ArticleGoogle Scholar
- Kwok EY, Hanson MR: In vivo analysis of interactions between GFP-labeled microfilaments and plastid stromules. BMC Plant Biol. 2004, 4: 2-10.1186/1471-2229-4-2.PubMedPubMed CentralView ArticleGoogle Scholar
- Sattarzadeh A, Krahmer J, Germain AD, Hanson MR: A Myosin XI Tail Domain Homologous to the Yeast Myosin Vacuole-Binding Domain Interacts with Plastids and Stromules in Nicotiana benthamiana. Mol Plant. 2009, 2 (6): 1351-1358. 10.1093/mp/ssp094.PubMedView ArticleGoogle Scholar
- Kwok EY, Hanson MR: Microfilaments and microtubules control the morphology and movement of non-green plastids and stromules in Nicotiana tabacum. Plant J. 2003, 35 (1): 16-26. 10.1046/j.1365-313X.2003.01777.x.PubMedView ArticleGoogle Scholar
- Waters MT, Fray RG, Pyke KA: Stromule formation is dependent upon plastid size, plastid differentiation status and the density of plastids within the cell. Plant J. 2004, 39 (4): 655-667. 10.1111/j.1365-313X.2004.02164.x.PubMedView ArticleGoogle Scholar
- Köhler RH, Hanson MR: Plastid tubules of higher plants are tissue-specific and developmentally regulated. J Cell Sci. 2000, 113 (Pt 1): 81-89.PubMedGoogle Scholar
- Marques JP, Dudeck I, Klosgen RB: Targeting of EGFP chimeras within chloroplasts. Mol Genet Genomics. 2003, 269 (3): 381-387. 10.1007/s00438-003-0846-y.PubMedView ArticleGoogle Scholar
- Marques JP, Schattat MH, Hause G, Dudeck I, Klosgen RB: In vivo transport of folded EGFP by the DeltapH/TAT-dependent pathway in chloroplasts of Arabidopsis thaliana. J Exp Bot. 2004, 55 (403): 1697-1706. 10.1093/jxb/erh191.PubMedView ArticleGoogle Scholar
- Natesan SKA, Sullivan JA, Gray JC: Myosin XI Is Required for Actin-Associated Movement of Plastid Stromules. Mol Plant. 2009, 2 (6): 1262-1272. 10.1093/mp/ssp078.PubMedView ArticleGoogle Scholar
- Dinant S, Lemoine R: The phloem pathway: New issues and old debates. Comptes Rendus Biologies. 2010, 333 (4): 307-319. 10.1016/j.crvi.2010.01.006.PubMedView ArticleGoogle Scholar
- Solfanelli C, Poggi A, Loreti E, Alpi A, Perata P: Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis. Plant Physiol. 2006, 140 (2): 637-646. 10.1104/pp.105.072579.PubMedPubMed CentralView ArticleGoogle Scholar
- Loreti E, Alpi A, Perata P: Glucose and disaccharide-sensing mechanisms modulate the expression of alpha-amylase in barley embryos. Plant Physiol. 2000, 123 (3): 939-948. 10.1104/pp.123.3.939.PubMedPubMed CentralView ArticleGoogle Scholar
- Luo Y, Liu YB, Dong YX, Gao XQ, Zhang XS: Expression of a putative alfalfa helicase increases tolerance to abiotic stress in Arabidopsis by enhancing the capacities for ROS scavenging and osmotic adjustment. J Plant Physiol. 2009, 166 (4): 385-394. 10.1016/j.jplph.2008.06.018.PubMedView ArticleGoogle Scholar
- Davletova S, Schlauch K, Coutu J, Mittler R: The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis. Plant Physiology. 2005, 139 (2): 847-856. 10.1104/pp.105.068254.PubMedPubMed CentralView ArticleGoogle Scholar
- Arce DP, Godoy AV, Tsuda K, Yamazaki K, Valle EM, Iglesias MJ, Di Mauro MF, Casalongue CA: The analysis of an Arabidopsis triple knock-down mutant reveals functions for MBF1 genes under oxidative stress conditions. J Plant Physiol. 2010, 167 (3): 194-200. 10.1016/j.jplph.2009.09.003.PubMedView ArticleGoogle Scholar
- Zhu JH, Lee BH, Dellinger M, Cui XP, Zhang CQ, Wu S, Nothnagel EA, Zhu JK: A cellulose synthase-like protein is required for osmotic stress tolerance in Arabidopsis. Plant Journal. 2010, 63 (1): 128-140.PubMedPubMed CentralGoogle Scholar
- Büttner M: The monosaccharide transporter(-like) gene family in Arabidopsis. Febs Letters. 2007, 581 (12): 2318-2324. 10.1016/j.febslet.2007.03.016.PubMedView ArticleGoogle Scholar
- Mulo P, Pursiheimo S, Hou CX, Tyystjarvi T, Aro EM: Multiple effects of antibiotics on chloroplast and nuclear gene expression. Functional Plant Biology. 2003, 30 (11): 1097--1103. 10.1071/FP03149.View ArticleGoogle Scholar
- Boulter D: Protein Synthesis in Plants. Annual Review of Plant Physiology. 1970, 21: 91-10.1146/annurev.pp.21.060170.000515.View ArticleGoogle Scholar
- Burns DJW, Cundliff E: Bacterial Protein Synthesis - Novel System for Studying Antibiotic Action in-Vivo. European Journal of Biochemistry. 1973, 37 (3): 570-574. 10.1111/j.1432-1033.1973.tb03020.x.PubMedView ArticleGoogle Scholar
- Moazed D, Noller HF: Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature. 1987, 327 (6121): 389-394. 10.1038/327389a0.PubMedView ArticleGoogle Scholar
- Etzold T, Fritz CC, Schell J, Schreier PH: A Point Mutation in the Chloroplast 16 S Rna Gene of a Streptomycin Resistant Nicotiana-Tabacum. Febs Letters. 1987, 219 (2): 343-346. 10.1016/0014-5793(87)80249-1.View ArticleGoogle Scholar
- Hanson J, Smeekens S: Sugar perception and signaling-an update. Curr Opin Plant Biol. 2009, 12 (5): 562-567. 10.1016/j.pbi.2009.07.014.PubMedView ArticleGoogle Scholar
- Smeekens S, Ma JK, Hanson J, Rolland F: Sugar signals and molecular networks controlling plant growth. Current Opinion in Plant Biology. 2010, 13 (3): 274-279.PubMedView ArticleGoogle Scholar
- Wingler A, Roitsch T: Metabolic regulation of leaf senescence: interactions of sugar signalling with biotic and abiotic stress responses. Plant Biology. 2008, 10: 50-62.PubMedView ArticleGoogle Scholar
- Sage TL, Sage RF: The functional anatomy of rice leaves: implications for refixation of photorespiratory CO2 and efforts to engineer C4 photosynthesis into rice. Plant Cell Physiol. 2009, 50 (4): 756-772. 10.1093/pcp/pcp033.PubMedView ArticleGoogle Scholar
- Hanson MR, Sattarzadeh A: Stromules: recent insights into a long neglected feature of plastid morphology and function. Plant Physiol. 2011.Google Scholar
- Holzinger A, Wasteneys GO, Lutz C: Investigating cytoskeletal function in chloroplast protrusion formation in the arctic-alpine plant Oxyria digyna. Plant Biol (Stuttg). 2007, 9 (3): 400-410. 10.1055/s-2006-924727.View ArticleGoogle Scholar
- Buchner O, Holzinger A, Lütz C: Effects of temperature and light on the formation of chloroplast protrusions in leaf mesophyll cells of high alpine plants. Plant Cell Environ. 2007, 30 (11): 1347-1356. 10.1111/j.1365-3040.2007.01707.x.PubMedView ArticleGoogle Scholar
- Rolland F, Baena-Gonzalez E, Sheen J: Sugar sensing and signaling in plants: conserved and novel mechanisms. Annu Rev Plant Biol. 2006, 57: 675-709. 10.1146/annurev.arplant.57.032905.105441.PubMedView ArticleGoogle Scholar
- Neuhaus HE, Emes MJ: Nonphotosynthetic metabolism in plastids. Annual Review of Plant Physiology and Plant Molecular Biology. 2000, 51: 111-140. 10.1146/annurev.arplant.51.1.111.PubMedView ArticleGoogle Scholar
- Gonzali S, Loreti E, Solfanelli C, Novi G, Alpi A, Perata P: Identification of sugar-modulated genes and evidence for in vivo sugar sensing in Arabidopsis. Journal of Plant Research. 2006, 119 (2): 115-123. 10.1007/s10265-005-0251-1.PubMedView ArticleGoogle Scholar
- Price J, Laxmi A, St Martin SK, Jang JC: Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis. Plant Cell. 2004, 16 (8): 2128-2150. 10.1105/tpc.104.022616.PubMedPubMed CentralView ArticleGoogle Scholar
- Yamada SY, Sonobe S, Shimmen T: Synthesis of a callosic substance during rhizoid differentiation in Spirogyra. Plant Cell Physiol. 2003, 44 (11): 1225-1228. 10.1093/pcp/pcg152.PubMedView ArticleGoogle Scholar
- Hadley A: My software to combine pictures to increase Depth of Field. 2006, [http://www.hadleyweb.pwp.blueyonder.co.uk/]Google Scholar
- Schattat MH, Klösgen RB: Improvement of plant cell microscope images by use of "Depth of Field" - extending software. Endocytobiosis Cell Res. 2009, 19: 11-19.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.