Figure 6From: Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responsesExpression of MtCBF4 in response to ABA, drought, salt and cold treatments. Four-week-old seedlings were subjected to the following treatments: (a) 200 μl ABA solution containing 0.05% Tween20 (v/v) was sprayed onto leaves for 1, 6, or 24 h; (b) For drought treatment, seedlings were transferred to dry Whatman 3 MM paper in a sterile Petri dish for 1, 2, or 3 h; (c) Seedlings were treated for 6, 24, or 48 h with 180 mM NaCl; (d) Seedlings were placed in a growth chamber at 4°C for 1, 6, or 24 h. The MtActin gene was amplified as a control. Data represent the mean and standard error (SE) for three replications. Primers used are listed in Additional file 3 (Table S1).Back to article page